文献类型：期刊文献

英文题名：Sedum alfredii SaNramp6 Metal Transporter Contributes to Cadmium Accumulation in Transgenic Arabidopsis thaliana

作者：Chen, Shuangshuang[1,2] Han, Xiaojiao[1,2] Fang, Jie[1,2,3] Lu, Zhuchou[4] Qiu, Wenmin[1,2] Liu, Mingying[1,2] Sang, Jian[1,2] Jiang, Jing[1,2] Zhuo, Renying[1,2]

第一作者：Chen, Shuangshuang

通信作者：Zhuo, RY[1];Zhuo, RY[2]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]State Key Lab Forest Genet & Breeding, Xiangshan Rd, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Zhejiang, Peoples R China;[3]Lishui Inst Agr Sci, Chem Biol Ctr, Lishui 323000, Zhejiang, Peoples R China;[4]Three Gorges Univ, Biotechnol Res Ctr China, Yichang 443002, Hubei, Peoples R China

年份：2017

卷号：7

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000413084800015)】；

基金：We thank the anonymous referees and the editor for the comments and suggestions that helped improve the manuscript. This work was supported by Basic Scientific Research Project of Nonprofit Central Research Institutions (No. CAFYBB2012040 and TGB2013008), National High-tech R&D Program of China No. 2013AA102701-3, the National Natural Science Foundation of China (No. 31200465), the National Key R&D Program of China (No. 2016YFD0800801) and the National Nonprofit Institute Research Grant of CAF (No. CAFYBB2012040, No. RISF2014010).

语种：英文

摘要：The plant natural resistance-associated macrophage protein (Nramp) family plays an important role in tolerance to heavy metal stress. However, few Nramps have been functionally characterized in the heavy metal-accumulating plant Sedum alfredii. Here, Nramp6 was cloned and identified from S. alfredii and its function analyzed in transgenic Arabidopsis thaliana. SaNramp6 cDNA contains an open reading frame of 1, 638 bp encoding 545 amino acids. SaNramp6's expression can be induced by cadmium (Cd) stress, and, after treatment, it peaked at one week and 12 h in the roots and leaves, respectively. SaNramp6 localized to the plasma membrane in protoplasts isolated from A. thaliana, Nicotiana benthamiana lower leaf and onion (Allium cepa) epidermal cells. The heterologous expression of SaNramp6 in the Delta ycf1 yeast mutant increased the Cd content in yeast cells. SaNramp6 also rescued the low Cd accumulation of the A. thaliana nramp1 mutant. Transgenic A. thaliana expressing SaNramp6 exhibited high Cd accumulation levels, as determined by a statistical analysis of the Cd concentration, translocation factors and net Cd2+ fluxes under Cd stress. Thus, SaNramp6 may play a significant role in improving Cd accumulation, and the gene may be useful for the biotechnological development of transgenic plants for phytoremediation.