文献类型：期刊文献

英文题名：PagWOX11/12a from hybrid poplar enhances drought tolerance by modulating reactive oxygen species

作者：Zuo, Wen-Teng[1] Meng, Jia-Hui[1] Liu, Hong-Chao[1] Zhu, Hang-Yong[4] Lu, Meng-Zhu[2,3] Wang, Liu- Qiang[1,2]

第一作者：Zuo, Wen-Teng

通信作者：Wang, LQ[1];Wang, LQ[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Peoples R China;[3]Zhejiang A&F Univ, Coll Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China;[4]Guangdong Acad Forestry, Guangdong Prov Key Lab Silviculture Protect & Util, Guangzhou 510520, Guangdong, Peoples R China

年份：2024

卷号：211

外文期刊名：PLANT PHYSIOLOGY AND BIOCHEMISTRY

收录：;Scopus(收录号：2-s2.0-85191456694);WOS:【SCI-EXPANDED(收录号:WOS:001237949800001)】；

基金：This work was supported by the STI 2030-Major Projects (No. 2023ZD0405602) and Fundamental Research Funds for the Central Non-profit Research Institution of CAF (No. CAFYBB2022QC001-1). We also thank the Scientific instrument Platform of State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry.

语种：英文

外文关键词：Antioxidant enzyme; Drought tolerance; PagWOX11/12a; Poplar; Reactive oxygen species

摘要：WOX11/12 is a homeobox gene of WOX11 and WOX12 in Arabidopsis that plays important roles in crown root development and growth. It has been reported that WOX11/12 participates in adventitious root (AR) formation and different abiotic stress responses, but the downstream regulatory network of WOX11/12 in poplar remains to be further investigated. In this study, we found that PagWOX11/12a is strongly induced by PEG-simulated drought stress. PagWOX11/12a -overexpressing poplar plantlets showed lower oxidative damage levels, greater antioxidant enzyme activities and reactive oxygen species (ROS) scavenging capacity than non-transgenic poplar plants, whereas PagWOX11/12a dominant repression weakened root biomass accumulation and drought tolerance in poplar. RNA-seq analysis revealed that several differentially expressed genes (DEGs) regulated by PagWOX11/12a are involved in redox metabolism and drought stress response. We used RT-qPCR and yeast onehybrid (Y1H) assays to validate the downstream target genes of PagWOX11/12a . These results provide new insights into the biological function and molecular regulatory mechanism of WOX11/12 in the abiotic resistance processes of poplar.