文献类型：期刊文献

英文题名：An Endophytic Diaporthe apiculatum Produces Monoterpenes with Inhibitory Activity against Phytopathogenic Fungi

作者：Song, Xiao-Yu[1] Wang, Huihua[2] Ren, Fei[3] Wang, Kaiying[1] Dou, Guiming[1] Lv, Xing[1] Yan, Dong-Hui[1] Strobel, Gary[4]

第一作者：Song, Xiao-Yu

通信作者：Yan, DH[1]

机构：[1]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Key Open Lab Forest Protect Natl Forestry & Grass, Beijing 100091, Peoples R China;[2]Beijing Vocat Coll Agr, Dept Food & Biol Engn, Beijing 102442, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China;[4]Montana State Univ, Dept Plant Sci, Bozeman, MT 59717 USA

年份：2019

卷号：8

期号：4

外文期刊名：ANTIBIOTICS-BASEL

收录：;Scopus(收录号：2-s2.0-85075591336);WOS:【SCI-EXPANDED(收录号:WOS:000506678800075)】；

基金：This research was funded by Fundamental Research Funds of CAF (No. CAFYBB2017MA010), the National Key R&D Program of China (No. 2017YFD0600102) and the National Natural Science Foundation of China (31370645).

语种：英文

外文关键词：endophytic fungus; Diaporthe apiculatum; Leucaena leucocephala; antifungal activity; inhibition; monoterpenes; (-)-4-terpineol

摘要：Volatile organic compounds (VOCs) from endophytic fungi are becoming a potential antibiotic resource. The inhibitive effects of VOCs produced by an endophytic fungus in Leucaena leucocephala were investigated on plant pathogens in this study. Using standard morphological methods and multigene phylogeny, the fungus was identified as Diaporthe apiculatum strain FPYF 3052. Utilizing a two- compartment Petri plate bioassay method, the VOCs from this fungus showed bioactivity ranging from 23.8% to 66.7% inhibition on eight plant pathogens within 24 hours. The SPME-GC/MS technique identified fifteen volatile compounds with dominant terpenoids gamma-terpinene (39.8%), alpha-terpinene (17.2%), and (-)-4-terpineol (8.4%) from the VOCs. Commercial alpha-terpinene, gamma-terpinene, and (-)-4-terpineol demonstrated inhibition on the tested pathogens at concentrations from 0.2 to 1.0 mu l/ml within 72 h in the bioassay system. The inhibition rates were from 28% to 100% percent using 1.0 mu l/ml within 48 h. (-)-4-Terpineol was the most active of the terpenoids causing up to 100% inhibition. The data illustrate that these monoterpenes play an important role in the inhibitive bioactivity of the VOCs of D. apiculatum FPYF 3052. Most importantly, (-)-4-terpineol is now for the first time, reported to have capability of strong antifungal activity and could be developed as an antibiotic substance.