文献类型：期刊文献

中文题名：土壤淹水和铜污染对杞柳形态及生理生化特性的影响

英文题名：Effects of flooding and copper stress on phenotypic and physiological characteristics of Salix integra seedlings

作者：曹旖旎[1,2] 蔡泽宇[1] 李晓刚[1] 张建锋[1] 陈光才[1]

第一作者：曹旖旎

机构：[1]中国林业科学研究院亚热带林业研究所,杭州311400;[2]南京林业大学,南京210037

年份：2019

卷号：38

期号：2

起止页码：548-557

中文期刊名：生态学杂志

外文期刊名：Chinese Journal of Ecology

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金项目(31470619);浙江省重点研发计划项目(2018C03047)资助

语种：中文

中文关键词：铜;淹水胁迫;杞柳;光合特性;生理代谢

外文关键词：copper;flooding stress;Salix integra;photosynthetic characteristics;physiological metabolism

分类号：S793.9;X53

摘要：柳树是重金属污染湿地土壤植物修复的潜力树种之一。本研究以杞柳一支笔(Salix integra Yizhibi)幼苗为对象,分析其在淹水30、60和90 d时的形态及生理生化响应。采用土壤培养模拟淹水试验,设置淹水(F,高于土壤表面10 cm)和不淹水(NF,正常土壤水分)处理及4个Cu浓度(0、50、150和450 mg·kg^(-1))。结果表明,淹水处理后,柳树茎基部产生了有利于吸收氧的白色皮孔和不定根来适应淹水环境,但高浓度(450 mg·kg^(-1)) Cu胁迫下只有极少量的不定根产生。随着淹水时间的延长,叶片光合色素和光合能力较不淹水处理明显下降,且随着Cu浓度的增加,光合速率减少了9.19%～44.48%。叶片SOD活性随着淹水时间的增加而升高,且SOD活性也随着Cu浓度的增加而升高; POD和CAT受淹水胁迫略有下降,但仍可保持一定活性,使杞柳适应水淹环境正常生长。随着淹水时间和Cu浓度的增加,大部分叶片MDA含量都呈现不同程度的增加。淹水60 d时,叶片MDA含量显著增加了8.52%～70.44%。淹水胁迫下,叶片超氧阴离子自由基含量变化与可溶性蛋白含量变化趋势相似,均降低。杞柳经较长时间的淹水后(除高浓度Cu处理)仍表现出较强的光合能力和生理适应性,表明其对土壤淹水和Cu污染的双重胁迫具有较强的耐性,在重金属污染湿地修复方面具有较大的应用潜力。
Willow is one of the suitable species for phytoremediation on heavy metal polluted soil in wetlands. In this study,we investigated the phenotypic and physiological responses of seedlings of Salix integra Yizhibi under flooding and Cu stress during different treatment periods. The willow plants were cultivated in Cu-polluted soils under the treatments of flooding( F,with water level of 10 cm above the soil surface) and non-flooded( NF,normal soil moisture). The Cu treatments were set as 0,50,150 and 450 mg·kg^-1of Cu spiked with CuSO4·5H2O. Under flooding condition,white lenticels and adventitious root emerged from stem base to adapt to the flooding environment,while only a few adventitious roots were found at high Cu concentration( 450 mg·kg^-1). Photosynthetic pigments and photosynthetic capacity substantially decreased as the flooding treatment prolonged. Photosynthetic rate( Pn) was reduced by 9.19%-44.48% with the increasing Cu concentration. Leaf superoxide dismutase( SOD) activity was elevated with the prolonged flooding period and the increased Cu levels. Peroxidase( POD) and catalase( CAT)activities were slightly decreased by soil flooding,which still maintained a certain activity to adapt to normal growth under flooding condition. With the prolonged flooding treatment and the elevated Cu level,the Malonaldehyde( MDA) content in leaves increased. MDA content in leaves significantly increased by 8.52%-70.44% when being flooded for 60 d. Under flooding condition,the concentrations of superoxide anion radical( O2-·) and soluble protein showed the similar decreasing tendency. Salix integra showed strong growth, photosynthetic and physiological adaptation to Cu polluted soil for a long-term flooded period( except for high Cu concentration),indicating that willow could adapt to Cu polluted wetland.