文献类型：期刊文献

英文题名：Effect of pretreatment severity on the inhibitory behaviors of larch lignins in enzymatic hydrolysis

作者：Wen, Li[1] Tan, Xin[2] Zhang, Daihui[3] Jia, Yuan[1] Sheng, Yequan[2] Lai, Chenhuan[1] Yong, Qiang[1]

第一作者：Wen, Li

通信作者：Lai, CH[1];Sheng, YQ[2]

机构：[1]Nanjing Forestry Univ, Coll Chem Engn, Jiangsu Co Innovat Ctr Efficient Proc & Utilizat F, Nanjing 210037, Peoples R China;[2]Anhui Polytech Univ, Coll Biol & Food Engn, Wuhu 241000, Anhui, Peoples R China;[3]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Nanjing 210042, Peoples R China

年份：2023

卷号：197

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20231413852980);Scopus(收录号：2-s2.0-85151525383);WOS:【SCI-EXPANDED(收录号:WOS:000981714900001)】；

基金：This work is supported by National Natural Science Foundation of China (31971606) , Natural Science Foundation of Jiangsu Province (BK20210623) , and Anhui Polytechnic University (2022YQQ069) .

语种：英文

外文关键词：Combined severity factor; Larch; Residual lignin; Enzymatic hydrolysis; NMR analysis; QCM analysis

摘要：To explore the underlying reasons for the decreased enzymatic hydrolysis yields of the acid pretreated softwood under the higher pretreatment severity, the residual bulk lignins, as one type lignin fraction with stronger in-hibition, were isolated from larch (softwood) pretreated with different pretreatment severity in this study. The lignin structure changes during the pretreatment were comprehensively investigated by nuclear magnetic resonance (NMR) analysis. The larch lignins addition decreased the enzymatic hydrolysis of Avicel from 68.43% to 25.9-39.3%. The pretreatment intensified the inhibition of isolated lignins on enzymatic hydrolysis of Avicel. Furthermore, quartz crystal microbalance (QCM) analysis was applied to investigate the interaction between enzyme and lignins, distinguishing the reversible and irreversible enzyme adsorption. It demonstrated that the obviously higher irreversible adsorption of enzyme was observed on lignins from pretreated substrates with the higher severity. Additionally, the correlation between structure and inhibition of residual lignins in the enzy-matic hydrolysis was then investigated with Pearson's correlation heatmap. The findings can be used to develop theoretical guidelines for alleviating the residual lignin inhibition in the enzymatic hydrolysis of acidic pre-treated softwood.