文献类型：期刊文献

英文题名：Isolation and characterization of phosphate-solubilizing bacterium Pantoea rhizosphaerae sp. nov. from Acer truncatum rhizosphere soil and its effect on Acer truncatum growth

作者：Ma, Qinghua[1,2] He, Shanwen[3] Wang, Xing[4] Rengel, Zed[5,6] Chen, Lin[1] Wang, Xinghong[1] Pei, Shunxiang[1] Xin, Xuebing[1] Zhang, Xiaoxia[4]

通信作者：Xin, XB[1];Zhang, XX[2]

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, Natl Permanent Scientifc Res Base Warm Temperate Z, Beijing, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[3]Shanghai Acad Landscape Architecture Sci & Plannin, Key Lab, Natl Forestry & Grassland Adm Ecol Landscaping Cha, Shanghai, Peoples R China;[4]Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, State Key Lab Efficient Utilizat Arid & Semiarid A, Beijing, Peoples R China;[5]Univ Western Australia, UWA Sch Agr & Environm, Perth, WA, Australia;[6]Inst Adriat Crops & Karst Reclamat, Split, Croatia

年份：2023

卷号：14

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85166638837);WOS:【SCI-EXPANDED(收录号:WOS:001037731600001)】；

基金：This study was supported by the Science Foundation of the Chinese Academy of Forestry (CAFYBB2020ZB005) and the Agricultural Science and Technology Innovation Program (ASTIP No. CAAS-ZDRW202201).

语种：英文

外文关键词：Pantoea; genome; phosphate-solubilizing bacteria; Acer truncatum; P accumulation

摘要：The Acer truncatum Bunge, widely distributed in North China, shows excellent tolerance to low-P soils. However, little information is available on potential phosphate-solubilizing bacterial (PSB) strains from the A. truncatum rhizosphere. The objectives of this work were to isolate and characterize PSB from A. truncatum rhizosphere soil and to evaluate the effect of inoculation with the selected strain on A. truncatum seedlings. The strains were characterized on the basis of phenotypic characteristics, carbon source utilization pattern, fatty acid methyl esters analysis, 16S rRNA gene and the whole-genome sequence. A Gram-negative and rod-shaped bacterium, designated MQR6(T), showed a high capacity to solubilize phosphate and produce indole-3-acetic acid (IAA) and siderophores. The strain can solubilize tricalcium phosphate (TCP) and rock phosphate (RP), and the solubilization of TCP was about 60% more effective than RP. Phylogenetic analyses based on the 16S rRNA gene and whole-genome sequences revealed that strain MQR6(T) formed a distinct phyletic lineage as a new species within the genus Pantoea. The digital DNA-DNA hybridization value between strain MQR6(T) and the closely related strains was 19.5-23.3%. The major cellular fatty acids were summed feature 3 (C-16:1 & omega;7c and/or C-16:1 & omega;6c), summed feature 8 (C-18:1 & omega;6c and/or C-18:1 & omega;7c), C-14:0, C-16:0, and C-17:0 cyclo. Several genes related to IAA production, phosphonate transport, phosphate solubilization and siderophore biogenesis were found in the MQR6(T) genome. Furthermore, inoculation with the strain MQR6(T) significantly improved plant height, trunk diameter, dry weight and P accumulation in roots and shoot of A. truncatum seedlings compared to non-inoculated control. These plant parameters were improved even further in the treatment with both inoculation and P fertilization. Our results suggested that MQR6(T) represented a new species we named Pantoea rhizosphaerae, as a plant growth-promoting rhizobacterium that can solubilize inorganic P and improve growth of A. truncatum seedlings, emerging as a potential strategy to improve A. truncatum cultivation.