详细信息
毛竹根部解磷细菌的筛选及多样性研究 被引量:15
Diversity of the phosphate solubilizing bacteria isolated from the root of Phyllostachys pubescens
文献类型:期刊文献
中文题名:毛竹根部解磷细菌的筛选及多样性研究
英文题名:Diversity of the phosphate solubilizing bacteria isolated from the root of Phyllostachys pubescens
作者:韩烁[1] 夏冬亮[1] 李潞滨[2] 韩继刚[1]
第一作者:韩烁
机构:[1]河北大学生命科学学院;[2]中国林业科学研究院林业研究所国家林业局林木培育重点实验室
年份:2010
卷号:33
期号:2
起止页码:26-31
中文期刊名:河北农业大学学报
外文期刊名:Journal of Agricultural University of Hebei
收录:CSTPCD;;北大核心:【北大核心2008】;CSCD:【CSCD_E2011_2012】;
基金:上海市科委重大攻关计划(09dz1205001)
语种:中文
中文关键词:毛竹;解磷细菌;多样性
外文关键词:Phyllostachys pubescens; phosphate-solubilizing bacteria; diversity
分类号:S795.7
摘要:本研究对毛竹(Phyllostachys pubescens)根部(包括根际、根面及内生细菌)解磷细菌进行了筛选,并通过16S rDNA序列分析对其多样性进行了初步研究。从179株毛竹根部细菌中筛选出26株解磷细菌,包括14株根际细菌、8株根面细菌、4株内生细菌。其中,芽孢杆菌BK24的解磷活性最高,其解磷活性达到369.06 mg/L。16S rDNA序列分析表明:26株解磷细菌中,13株属于厚壁菌门(Firmicutes,50.00%),5株属于变形菌门β亚群(Betaproteobacteria,19.23%),4株属于变形菌门γ亚群(Gammaproteobacteria,15.38%),4株属于放线菌门(Actinobacteria,15.38%)。芽孢杆菌属(Bacillus,38.46%)和伯克霍尔德氏菌属(Burkholderia,19.23%)为优势菌属。
26 phosphate-solubilizing bacteria were screened from 179 rhizosphere,rhizoplane and root endophytic bacterial strains of moso bamboo(Phyllostachys pubescens) plants.The phosphate-solubilizing bacteria included fourteen rhizobacteria isolates,eight rhizoplane bacteria isolates,and four endophytic bacteria isolates.Bacillus sp.BK24 showed the highest phosphate solubilizing activity(369.06 mg/L).Phylogenetic analysis based on the partial 16S rDNA sequences indicated that the strains were clustered into four groups: thirteen strains of Firmicutes(50.00%),five strains of Betaproteobacteria(19.23%),four strains of Gammaproteobacteria(15.38%) and four strains of Actinobacteria(15.38%).The dominant phosphate-solubilizing bacteria were Bacillus and Burkholderia,which was 38.46% and 19.23% respectively.
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