文献类型：期刊文献

中文题名：缓释肥×菌根菌对赤皮青冈容器苗生长性状和养分状况的影响

英文题名：Effects of Slow-release Fertilizer Addition and Inoculation of Mycorrhizal Fungi on the Growth Traits and Nutrient Status of Cyclobalanopsis gilva Container Seedlings

作者：黄盛怡[1] 杨孟晴[1] 王斌[1] 周志春[1] 王秀花[2] 徐卫可[2] 吴仁超[2]

第一作者：黄盛怡

机构：[1]中国林业科学研究院亚热带林业研究所,浙江省林木育种技术研究重点实验室,浙江杭州311400;[2]浙江省庆元县实验林场,浙江庆元323800

年份：2022

卷号：35

期号：6

起止页码：161-169

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2020SY015);浙江省省院合作林业科技项目(2021SY04);江西省林业科技创新专项项目(201803);浙江省科技计划项目(2021C02038)。

语种：中文

中文关键词：赤皮青冈;容器苗;缓释肥;菌根菌;生长性状;养分状况

外文关键词：Cyclobalanopsis gilva;container seedling;slow-release fertilizer;mycorrhizal fungi;growth traits;nutrient status

分类号：S723.8

摘要：[目的]基于不同缓释肥添加梯度和接种菌根菌互作试验,分析缓释肥添加和接种菌根菌对赤皮青冈1年生容器苗生长性状和养分状况的作用规律,以提高赤皮青冈容器苗的菌根化育苗水平。[方法]采用裂区试验设计,主区为菌根菌处理,分为接种(育苗容器中间部位注射浓度为36.28 mg·mL^(?1)的彩色豆马勃10 mL)与不接种2个处理;副区为基质缓释肥添加处理,设置5个缓释肥添加梯度(1.50、2.25、3.00、3.75和4.50 kg·m^(?3))。[结果]接种菌根菌对不同缓释肥梯度下1年生赤皮青冈容器苗生长均有促进作用,缓释肥添加和接种菌根菌对容器苗总生物量和P利用指数有显著的互作效应。接种菌根菌后,赤皮青冈容器苗苗高、地径、高径比、总生物量、总根长和根系直径等生长性状相比不接种分别增加18.70%、7.16%、10.75%、24.55%、1.02%和18.47%,N含量、N吸收量和N利用指数、P吸收量和P利用指数分别增加2.04%、27.10%、27.67%、19.72%和30.52%,P含量降低2.94%,除总根长和N、P含量外各生长性状和养分状况指标接种和不接种存在极显著差异。无论是否接种菌根菌,随缓释肥添加量增加,除根系直径无明显变化外各生长性状均呈先增加后降低趋势,P含量和P吸收量整体呈增加趋势,在缓释肥添加量为3 kg·cm^(?3)时生长表现最好。不接种菌根菌容器苗N含量和N吸收量呈先增加后降低趋势,N、P利用指数呈先降低后增加趋势,接种菌根菌后N、P利用指数变化趋势正好相反,呈先增加后降低趋势,N含量和N吸收量无明显变化规律。[结论]接种外生菌根菌彩色豆马勃可促进赤皮青冈容器苗的生长,提高N、P养分利用效率,低缓释肥添加量下菌根的促生效应更显著。生产中可通过接种菌根菌提高容器苗质量,同时减少缓释肥使用量,有助于培育赤皮青冈优质苗木并节约育苗成本。
[Objective]To improve the container seedlings cultivation level of Cyclobalanopsis gilva with mycorrhiza.[Method]Based on the interaction experiments of different slow-release fertilizer addition levels and inoculation of mycorrhizal fungi,the effects of slow-release fertilizer×mycorrhizal fungi on the growth traits and nutrient status of 1-year-old container seedlings of Cyclobalanopsis gilva were analyzed.A split-plot experimental design was used with inoculation of mycorrhizal fungi in the main plot and different slow-release fertilizer addition levels in the sub-plot.The main plot was mycorrhizal treatment,which was divided into two treatments:inoculation(10 mL of Pisolithus tinctorius with a concentration of 36.28 mg·mL^(?1) was injected into the middle part of the seedling container)and non inoculation.The area was treated with matrix slow-release fertilizer addition,and five slow-release fertilizer addition gradients(1.50,2.25,3.00,3.75,and 4.50 kg·m^(?3))were set.[Results]Inoculation of mycorrhizal fungi promoted the growth of 1-year-old C.gilva container seedlings under different slow-release fertilizer addition levels.There was significant interaction between slow-release fertilizer addition and inoculation of mycorrhizal fungi on total biomass and P utilization index of container seedlings.After inoculation with mycorrhizal fungi,growth traits such as seedling height,caliper,sturdiness quotient,total biomass,total root length and root diameter increased by 18.70%,7.16%,10.75%,24.55%,1.02%and 18.47%compared with that no inoculation,respectively.N content,N uptake and utilization index,P uptake and utilization index increased by 2.04%,27.10%,27.67%,19.72%and 30.52%,respectively,while P content decreased by 2.94%.There were extremely significant differences in growth traits and nutrient status between inoculated and non-inoculated except total root length and N and P contents.Regardless of whether mycorrhizal fungi were inoculated or not,with the increase of slow-release fertilizer addition level,all growth traits increased first and then decreased except root diameter,P content and uptake showed an overall increasing trend.The growth was the best when the slow-release fertilizer addition level was 3 kg·cm^(?3).The N content and absorption of the container seedlings without mycorrhizal inoculation showed a trend of increasing first and then decreasing,while the N and P utilization indexes decreased first and then increased.There was no obvious change in N content and N absorption.[Conclusion]Inoculation of ectomycorrhizal fungus with P.tinctorius can promote the growth of C.gilva container seedlings and improve the nutrient utilization efficiency of N and P,and the effect of promoting mycorrhizal growth is more significant under low slow-release fertilizer addition..In production,inoculation of mycorrhizal fungi can improve the quality of container seedlings and reduce the use of slow-release fertilizer,which is helpful to the cultivation of high quality seedlings of C.gilva and save seedling cost.