文献类型：期刊文献

英文题名：Improved Tolerance of Teak (Tectona grandis L.f.) Seedlings to Low-Temperature Stress by the Combined Effect of Arbuscular Mycorrhiza and Paclobutrazol

作者：Zhou, Zaizhi[1] Ma, Huaming[1] Liang, Kunnan[1] Huang, Guihua[1] Pinyopusarerk, Khongsak[1,2]

第一作者：周再知

通信作者：Zhou, ZZ[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[2]CSIRO Plant Ind, Black Mt Labs, Canberra, ACT 2601, Australia

年份：2012

卷号：31

期号：3

起止页码：427-435

外文期刊名：JOURNAL OF PLANT GROWTH REGULATION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000307269100015)】；

基金：This research was supported by the research project on teak cultivation techniques (2012BAD21B01) and National Natural Science Foundation Project of China (30471382). The isolate of G. versiforme Gv9004 was kindly provided by the Mycorrhizae Research Group, Research Institute of Tropical Forestry, Chinese Academy of Forestry.

语种：英文

外文关键词：Teak (Tectona grandis); Seedlings; Arbuscular mycorrhiza; Paclobutrazol; Chilling stress; Physiological and biochemical response

摘要：Low-temperature damage is a common problem for tropical and subtropical plants during their early-growth stage. In this study, an experiment with a L-18 (2(1) x 3(7)) mixed orthogonal array in a greenhouse was conducted to determine whether arbuscular mycorrhizal fungi (AMF) inoculation and paclobutrazol (PBZ) application through foliar spray would enhance the chilling tolerance of teak seedlings. One-month-old seedlings of clones 8301, 7544, and 7552 from a Myanmar provenance propagated by tissue culture techniques were inoculated with Glomus versiforme and cultivated for 6 months. The foliar surface of both mycorrhizal and nonmycorrhizal treated plants was sprayed with PBZ at concentrations of 0, 50, and 100 mg l(-1) once a week for 3 weeks prior to exposure to low temperatures of 6, 3, and 0A degrees C for 12 h in an artificial climate chamber, followed by 12 h of recovery at 20A degrees C room temperature. AMF colonization significantly promoted height and RCD growth and dry biomass accumulation of shoot and root. Under low-temperature stress, AM symbiosis increased leaf chlorophyll content by 22.8%, soluble protein content by 19.6%, superoxide dismutase (SOD) activity by 10.6%, and peroxidase (POX) activity by 9.5%, whereas malondialdehyde content was decreased by 14.1%. Both AMF colonization and the foliar spray PBZ at 50 and 100 mg l(-1) were capable of alleviating the damage caused by low-temperature stress on teak seedlings by increasing the photosynthetic pigments, accumulation of osmotic adjustment compounds, and antioxidant enzyme (SOD and POX) activity, and by decreasing membrane lipid peroxidation. AMF colonization and foliar spraying of PBZ at 50 mg l(-1) produced a positive interaction and appears to be a good way to enhance chilling tolerance of teak seedlings experiencing stress at 6, 3 and 0A degrees C for 12 h.