文献类型：期刊文献

中文题名：中间锦鸡儿4个Fad2蛋白的比较与分析

英文题名：Comparison and Analysis of Four Fad2 Proteins in Caragana intermedia

作者：林萍[1] 汪阳东[2] 齐力旺[1] 王建华[1] 张守攻[1]

机构：[1]中国林业科学研究院林业研究所;[2]中国林业科学研究院亚热带林业研究所

年份：2008

卷号：16

期号：3

起止页码：486-493

中文期刊名：农业生物技术学报

外文期刊名：Journal of Agricultural Biotechnology

收录：CSTPCD;;CSCD:【CSCD2011_2012】；

基金：国家科技支撑计划项目(No.2006BAD01A1606)资助

语种：中文

中文关键词：中间锦鸡儿;fad2基因;磷酸化;棕榈酰化

外文关键词：Caragana intermedia; fad2 gene; phosphorylation palmitoylation

分类号：S188

摘要：对中间锦鸡儿(Caragana intermedia)4个fad2基因编码的4个蛋白质进行生物信息学分析,结果表明,4个蛋白中只有Fad2-2B有信号肽,其余3个蛋白都没有信号肽剪切位点。Fad2-2A和Fad2-1A二级结构相似,以无规卷曲为主,含少量直链,间或有α螺旋;Fad2-2B和Fad2-1B相似,主要以无规卷曲和直链为主。Fad2-2A蛋白半衰期是220h,其余蛋白的半衰期仅为30h或31h;蛋白翻译相对效率Fad2-2B为1,其余都是5。Fad2-1A和Fad2-1B的棕榈酰化位点分别是5个和4个,Fad2-2A和Fad2-2B的棕榈酰化位点分别是8个和9个。4个Fad2蛋白信号肽分析、蛋白质二级结构、磷酸化位点数量、稳定性和蛋白翻译效率、棕榈酰化位点数量等都有所不同,为进一步研究基因分工、调控方式,以及为实现对锦鸡儿属植物脂肪酸成分直接、精确调控提供了信息。
Four Fad2 proteins were analyzed by bioinformatics methods, and the results showed that Fad2-2B had signal peptide and the three others had no signal peptide cleaved sites. The secondary structure of Fad2-2A and Fad2-1A was similar, with main coil and little strand and helix. The Fad2-2B and Fad2-1B secondary structure was coil and strand mainly. The half life of Fad2-2A protein was 220 h, and that of the others were 30 or 31 h. Fad2-2B relative translation effectiveness was 1 and that of the other proteins were 5. The palmitoylation sites of Fad2-1A, Fad2-1B, Fad2-2A and Fad2-2B were 5, 4, 8 and 9, respectively. The signal pepfide, protein secondary structure, the amounts of phosphorylation sites and palmitoylation sites were different in the four C. intermedia Fad2 proteins. All of these are very important informations to study the functions and regulation of these genes further.