文献类型：期刊文献

英文题名：Genome-Wide Identification and Expression Analysis of Nitrate Transporter (NRT) Gene Family in Eucalyptus grandis

作者：Li, Guangyou[1] Yang, Deming[1] Hu, Yang[2] Xu, Jianmin[1] Lu, Zhaohua[1]

第一作者：李光友

通信作者：Lu, ZH[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, State Forestry & Grassland Adm Trop Forestry, Key Lab, Guangzhou 510520, Peoples R China;[2]Xinhui Res Inst Forestry Sci, Jiangmen 529100, Peoples R China

年份：2024

卷号：15

期号：7

外文期刊名：GENES

收录：;Scopus(收录号：2-s2.0-85199888607);WOS:【SCI-EXPANDED(收录号:WOS:001278866900001)】；

基金：This work was funded by the Fundamental Research Funds for the Central Non-Profit Research Institution of CAF (Nos. CAFYBB2022SY017 and CAFYBB2021SY001) and the National Key Research and Development Program of China during the 14th five-year plan Period (2023YFD2201003and 2022YFD2200203).

语种：英文

外文关键词：Eucalyptus grandis; nitrogen; EgNRTs; RNA-Seq; expression profile; nitrate deficiency

摘要：Eucalyptus grandis is an important planted hardwood tree worldwide with fast growth and good wood performance. The nitrate transporter (NRT) gene family is a major core involved in nitrogen (N) absorption and utilization in plants, but the comprehensive characterization of NRT genes in E. grandis remains mostly elusive. In this study, a total of 75 EgNRT genes were identified from the genome of E. grandis that were distributed unevenly across ten chromosomes, except Chr9. A phylogenetic analysis showed that the EgNRT proteins could be divided into three classes, namely NRT1, NRT2 and NRT3, which contained 69, 4 and 2 members, respectively. The cis-regulatory elements in the promoter regions of EgNRT genes were mainly involved in phytohormone and stress response. The transcriptome analysis indicated that the differentially expressed genes of leaf and root in E. grandis under different N supply conditions were mainly involved in the metabolic process and plant hormone signal transduction. In addition, the transcriptome-based and RT-qPCR analysis revealed that the expression of 13 EgNRT genes, especially EgNRT1.3, EgNRT1.38, EgNRT1.39 and EgNRT1.52, was significantly upregulated in the root under low-N-supply treatment, suggesting that those genes might play a critical role in root response to nitrate deficiency. Taken together, these results would provide valuable information for characterizing the roles of EgNRTs and facilitate the clarification of the molecular mechanism underlying EgNRT-mediated N absorption and distribution in E. grandis.