文献类型：期刊文献

英文题名：Genomics insights into flowering and floral pattern formation: regional duplication and seasonal pattern of gene expression in Camellia

作者：Hu, Zhikang[1,2,3] Fan, Zhengqi[1,3] Li, Sijia[1,2,3] Wang, Minyan[1,3] Huang, Mingchuan[1,4] Ma, Xianjin[1,3] Liu, Weixin[1,3] Wang, Yupeng[2] Yu, Yifan[1,2,3] Li, Yaxuan[1,2,3] Sun, Yingkun[4] Li, Xinlei[1,3] Li, Jiyuan[1,3] Yin, Hengfu[1,3]

第一作者：Hu, Zhikang

通信作者：Yin, HF[1];Yin, HF[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[2]Nanjing Forestry Univ, Coll Informat Sci & Technol, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Forest Genet & Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[4]Qingdao Agr Univ, Coll Hort, Qingdao 266109, Shandong, Peoples R China

年份：2024

卷号：22

期号：1

外文期刊名：BMC BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001177519000004)】；

基金：We would like to thank Dr. Y.D. Wang (Chinese Academy of Forestry) for critical comments on this work.

语种：英文

外文关键词：Camellia japonica; Genome; Ornamental traits; Annual gene expression; Genome duplication

摘要：Background The formation and domestication of ornamental traits are influenced by various aspects, such as the recognition of esthetic values and cultural traditions. Camellia japonica is widely appreciated and domesticated around the world mainly due to its rich variations in ornamental traits. Ornamental camellias have a diverse range of resources, including different bud variations from Camellia spp. as well as inter- and intra- specific hybridization. Despite research on the formation of ornamental traits, a basic understanding of their genetics and genomics is still lacking. Results Here, we report the chromosomal-level reference genome of C. japonica through combining multiple DNA-sequencing technologies and obtain a high-density genetic linkage map of 4255 markers by sequencing 98 interspecific F-1 hybrids between C. japonica and C. chekiangoleosa. We identify two whole-genome duplication events in C. japonica: one is a shared ancient gamma event, and the other is revealed to be specific to genus Camellia. Based on the micro-collinearity analysis, we find large-scale segmental duplication of chromosome 8, resulting to two copies of the AGAMOUS loci, which may play a key role in the domestication of floral shapes. To explore the regulatory mechanisms of seasonal flowering, we have analyzed year-round gene expression patterns of C. japonica and C. azalea-a sister plant of continuous flowering that has been widely used for cross breeding. Through comparative analyses of gene co-expression networks and annual gene expression patterns, we show that annual expression rhythms of some important regulators of seasonal growth and development, including GIGANTEA and CONSTANS of the photoperiod pathway, have been disrupted in C. azalea. Furthermore, we reveal that the distinctive expression patterns of FLOWERING LOCUS T can be correlated with the seasonal activities of flowering and flushing. We demonstrate that the regulatory module involved in GIGANTEA, CONSTANS, and FLOWERING LOCUS T is central to achieve seasonality. Conclusions Through the genomic and comparative genomics characterizations of ornamental Camellia spp., we propose that duplication of chromosomal segments as well as the establishment of gene expression patterns has played a key role in the formation of ornamental traits (e.g., flower shape, flowering time). This work provides a valuable genomic platform for understanding the molecular basis of ornamental traits.