文献类型：期刊文献

英文题名：Integrative analysis of transcriptome and proteome revealed nectary and nectar traits in the plant-pollinator interaction of Nitraria tangutorum Bobrov

作者：Chen, Tingting[1] Zhou, Yanwei[1] Zhang, Jingbo[2] Peng, Ye[3] Yang, Xiuyan[4] Hao, Zhaodong[1] Lu, Ye[1] Wu, Weihuang[1] Cheng, Tielong[3] Shi, Jisen[1] Chen, Jinhui[1]

第一作者：Chen, Tingting

通信作者：Chen, JH[1]

机构：[1]Nanjing Forestry Univ, Key Lab Forest Genet & Biotechnol, Minist Educ China, Coinnovat Ctr Sustainable Forestry Southern China, 159 Longpan Rd, Nanjing 210037, Peoples R China;[2]Chinese Acad Forestry, Expt Ctr Desert Forestry, Dengkou, Inner Mongolia, Peoples R China;[3]Nanjing Forestry Univ, Coll Biol & Environm, Nanjing 210037, Peoples R China;[4]Chinese Acad Forestry, Res Ctr Saline & Alkali Land Natl Forestry & Gras, Beijing 100091, Peoples R China

年份：2021

卷号：21

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-85106722020);WOS:【SCI-EXPANDED(收录号:WOS:000657653900003)】；

基金：This work was supported by the Key Research and Development Plan of Jiangsu Province (BE2017376), Nature Science Foundation of China (31770715, 32071784), the Qinglan Project of Jiangsu Province, Distinguished Professor Project of Jiangsu province and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

语种：英文

外文关键词：Ecological plant; Entomophilous; Nectary development; Antibacterial protein

摘要：Background Nitraria tangutorum is an important desert shrub that shows resistance to drought, salt and wind erosion stresses. It is a central ecological species in its area. Here, we have studied how N. tangutorum has adapted to achieve a successful reproduction strategy. Results We found that N. tangutorum is mainly pollinated by insects of the Hymenoptera, Diptera and Coleoptera orders. Nitraria tangutorum has very small flowers, with the nectary composed of secretive epidermal cells from which nectar is secreted, located within the inner petals. In addition, analyzing the transcriptome of four successive flower developmental stages revealed that mainly differentially expressed genes associated with flower and nectary development, nectar biosynthesis and secretion, flavonoid biosynthesis, plant hormone signal transduction and plant-pathogen interaction show dynamic expression. From the nectar, we could identify seven important proteins, of which the L-ascorbate oxidase protein was first found in plant nectar. Based on the physiological functions of these proteins, we predict that floral nectar proteins of N. tangutorum play an important role in defending against microbial infestation and scavenging active oxygen. Conclusions This study revealed that N. tangutorum is an insect-pollinated plant and its nectary is composed of secretive epidermal cells that specialized into secretive trichomes. We identified a large number of differentially expressed genes controlling flower and nectary development, nectar biosynthesis and secretion, flavonoid biosynthesis, plant hormone signal transduction and plant-pathogen interaction. We suggest that proteins present in N. tangutorum nectar may have both an antibacterial and oxygen scavenging effect. These results provide a scientific basis for exploring how the reproductive system of N. tangutorum and other arid-desert plants functions.