文献类型：期刊文献

英文题名：Metabolomics reveals significant variations in metabolites and correlations regarding the maturation of walnuts (Juglans regia L.)

作者：Rao, Guodong[1,2,3] Sui, Jinkai[2] Zhang, Jianguo[1,2,3]

第一作者：饶国栋;Rao, Guodong

通信作者：Zhang, JG[1];Zhang, JG[2];Zhang, JG[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing 210037, Jiangsu, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China

年份：2016

卷号：5

期号：6

起止页码：829-836

外文期刊名：BIOLOGY OPEN

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000378109400019)】；

基金：This work was supported by grants from the National Natural Science Foundation of China [31400569], the Fundamental Research Funds for the Central Non-profit Research Institution of CAF [CAFYBB2014QB028], Collaborative Innovation Plan of Jiangsu Higher Education [2013-2015], the Fundamental Research Funds for the Central Non-profit Research Institution of CAF [RIF2013-11].

语种：英文

外文关键词：Metabolome; Walnut kernel; Developmental stages; ANOVA; Metabolite-metabolite correlation

摘要：The content of walnut metabolites is related to its nutritive value and physiological characteristics, however, comprehensive information concerning the metabolome of walnut kernels is limited. In this study we analyzed the metabolites of walnut kernels at five developmental stages from filling to ripening using GC-MS-based untargeted metabolomics; of a total 252 peaks identified, 85 metabolites were positively identified. Further statistical analysis revealed that these 85 metabolites covered different types of metabolism pathways. PCA scores revealed that the metabolic compositions of the embryo are different at each stage, while the metabolic composition of the endotesta could not be significantly separated into distinct groups. Additionally, 7225 metabolite-metabolite correlations were detected in walnut kernel by a Pearson correlation coefficient approach; during screening of the calculated correlations, 463 and 1047 were determined to be significant with r(2)>= 0.49 and had a false discovery rate (FDR) <= 0.05 in endotesta and embryo, respectively. This work provides the first comprehensive metabolomic study of walnut kernels and reveals that most of the carbohydrate and protein-derived carbon was transferred into other compounds, such as fatty acids, during the maturation of walnuts, which may potentially provide the basis for further studies on walnut kernel metabolism.