文献类型：期刊文献

中文题名：辣椒感染疫病后生化指标的响应研究

英文题名：Study on Several Biochemical Indexes of Different Resistant Peppers after Infected with Phytophthora capsici

作者：马海宾[1] 郑服丛[2] 康丽华[1]

第一作者：马海宾

机构：[1]中国林科院热带林业研究所;[2]华南热带农业大学植保学院

年份：2006

卷号：16

期号：3

起止页码：35-38

中文期刊名：生物技术

外文期刊名：Biotechnology

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

基金：中国热带农业科学院基金项目("辣椒抗疫病的机制研究")

语种：中文

中文关键词：辣椒;辣椒疫霉;可溶性总糖;可溶性蛋白;保护酶

外文关键词：Capsicum annum ; Phytophthora capsici ; soluble sugar; soluble protein; protective enzyme

分类号：S432

摘要：采用生理生化分析方法研究了辣椒感染疫病后叶片中几个生化指标的变化。结果表明,染病前后感病品种叶片中可溶性总糖含量持续高于抗病品种;抗病类型品种和感病类型品种的可溶性蛋白含量变化规律均表现为先升高后下降,但接种前其叶片中可溶性蛋白含量两者间无明显差异;抗病类型和感病类型辣椒接种后保护酶活性均升高,而且感病类型的POD和ASP酶活性在接种后120h显著高于抗病类型;高抗类型叶片中PPO活性增加幅度显著大于感病类型,但抗病类型品种PPO活性上升趋势比较平缓。接种后,感病品种PAL活性上升幅度小于高抗品种,接种后96h PAL活性开始逐渐下降。可溶性总糖含量和苯丙氨酸解氨酶可以作为辣椒苗期抗疫病鉴定的生化指标。
The experiment result showed that, not only in healthy plants but in the infected, the content of soluble sugars was higher in susceptible cultivars than that in resistant ones. It was very apparent that the more resistant the cultivars, the higher the content of soluble protein. The content of soluble protein reached the peak at 96^th hour after inoculation. The results showed that the activities of POD and ASP in healthy leaves were significantly higher in resistant cultivars than those in susceptible ones. The activities increased both in resistant and in susceptible cultivars after inoculation. The activities were significantly higher in susceptible cultivars than those in the resistant during 96^th - 120^th after inoculation. The PPO activities increased both in resistant and in susceptible cultivars after inoculation, and their peaks were higher in highly resistant cultivars than those in the susceptible. However, the activities didn＇t increased sharply in resistant and medium resistant cuhivars. The PAL activities increased greatly both in resistant and in susceptible cultivars after inoculation. At 96^th hour after inoculation the PAL activities reached the peaks, which were lower in susceptible cultivars than those in the resistant.