文献类型：期刊文献

中文题名：杨树溃疡病拮抗链霉菌的筛选及鉴定

英文题名：Screening and Identification of Antagonistic Streptomyces for Biocontrol of Poplar Canker

作者：陈越渠[1,2] 刘庆珍[2] 李立梅[2] 张杨[2] 韩姣[2] 张永安[1]

第一作者：陈越渠

通信作者：Zhang, Yong'an

机构：[1]中国林业科学研究院森林生态环境与保护研究所国家林业和草原局森林保护学重点实验室,北京100091;[2]吉林省林业科学研究院,长春130033

年份：2021

卷号：57

期号：7

起止页码：92-100

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;EI(收录号：20213710886852);Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：吉林省科技厅技术攻关项目(20190301043NY);林业科技创新平台运行补助项目(2020132032);吉林省重点实验室(20170622017JC)。

语种：中文

中文关键词：杨树溃疡病;链霉菌;生物防治;分类鉴定

外文关键词：poplar canker;Streptomyces;biological control;screening and identification

分类号：S432.1

摘要：【目的】以生物防治防控杨树溃疡病发生与危害为目标,筛选能有效抑制杨树溃疡病且具有开发潜力的拮抗菌株,为杨树溃疡病的生物防治提供新的生防因子。【方法】从吉林省长白山国家级自然保护区横山保护站林下土壤样品中筛选获得1株对杨树溃疡病菌有良好抑制效果的链霉菌,编号为HS1。采用多重筛选法确定菌株HS1及其发酵液的抑菌活性,通过形态学、生理生化特征、16S rDNA序列分析等方法明确其分类地位,运用人工接种法测定菌株HS1发酵液对杨树溃疡病的预防和治疗效果。【结果】菌株HS1及其发酵液对供试植物病原菌均有抑制作用,抑菌谱广,其中对杨树溃疡病病原菌抑制作用最强,抑菌带宽达20.66 mm,发酵液抑菌圈直径达40.26 mm;16S rDNA序列比对分析发现,菌株HS1与Streptomyces scopuliridis同源性最高;人工接种试验结果表明,菌株HS1发酵液对杨树溃疡病的预防和治疗效果分别为60.87%和71.74%。【结论】生防菌株HS1可有效预防和控制杨树溃疡病,且对多种农林重要病害病原菌的抑菌作用效果明显,具有广阔的开发应用前景。
【Objective】In order to control the poplar canker disease by biological control agent,the present study aimed to screen and obtain antagonistic strains from Streptomyces that can effectively inhibit poplar canker.【Method】A streptomyces strain with good biocontrol effect against the pathogen of poplar canker was obtained from the soil in the larch forest Hengshan Protection Station,Changbaishan Mountain National Nature Reserve,Jilin Province.The number was recorded as HS1.Multiple screening was used to determine the antibacterial activity of the strain and its fermentation broth;morphological,physiological and biochemical characteristics,16S rDNA sequence analysis were used to determine its taxonomic status;artificial inoculation method was used to determine the preventive and therapeutic effects of HS1 fermentation broth on poplar canker.【Result】The result showed that strain HS1 and its fermentation broth had inhibitory effect on the tested plant pathogens,and the antibacterial spectrum was relatively wide.Among them,it had the strongest inhibitory effect on Botryosphaeria dothidea.The width of inhibition zone averagely was more than 20.66 mm and the diameter of the bacteriostatic circle of the fermentation broth reached 40.26 mm.The 16S rDNA sequence alignment analysis showed that the strain had the highest homology with Streptomyces scopuliridis;The result of artificial inoculation test showed that the preventive and therapeutic effects of strain HS1 fermentation broth on poplar canker were 60.87%and 71.74%respectirly.【Conclusion】The screened strain HS1 and its fermentation broth could effectively prevent and inhibit the occurrence and expansion of poplar canker disease,and also have a good antibacterial effect on other pathogens of many important agricultural and forest diseases.Therefore,it has a good for development and application prospects in biocontrol of forest pathogens.