文献类型：期刊文献

中文题名：杨树叶片数量性状相关联标记及其图谱定位研究

英文题名：DETECTION AND IDENTIFICATION OF MOLECULAR MARKERS ASSOCIATED WITH QUANTITATIVE TRAITS OF LEAF IN POPLAR

作者：苏晓华[1] 李金花[1] 陈伯望[1] 张绮纹[1] 张香华[1]

第一作者：苏晓华

机构：[1]中国林业科学研究院林业研究所

年份：2000

卷号：36

期号：1

起止页码：33-40

中文期刊名：林业科学

外文期刊名：SCIENTIA SILVAE SINICAE

收录：CSTPCD;;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：林业部指南项目;国家科技部"九五"攀登项目

语种：中文

中文关键词：杨树;数量性状;关联标记;图谱定位;叶片

外文关键词：Poplar, Quantitative traits, Associated markers, Identification

分类号：S792.110.1

摘要：利用数量遗传学方法和分子标记相结合，对美洲黑杨与青杨杂交产生的Ｆ２ 群体５ 个叶片数量性状进行了相关联标记及其图谱定位研究。用单因素方差分析检测出与叶长、叶宽、叶长／ 宽比、叶柄长和叶面积相关联的ＲＡＰＤ 标记分别为１０ ，１０ ，４ ，９ ，１２ 个；联合贡献率分别为６６－２３ ％ ，６１－８２ ％ ，３２－８６ ％ ，５９－６７ ％ ，８１－７９ ％ 。用双因素方差分析检测出与互作ＱＴＬｓ 紧密相关联的标记位点共１９ 对，其中与叶长、叶宽、叶长／ 宽比、叶柄长和叶面积显著相关联的标记分别有４ 对，２ 对，１ 对，５ 对，７ 对。与这５ 个性状相关联的标记多数集中分布于第４ ，１２ ，１５ 和１７ 条连锁群上。
Both quantitative genetics method and molecular marker method were used to identify marker loci associated with 5 quantitative leaf traits in a F\-2\|population from a cross of \%Populus deltoides\% Marsh. and \%P. cathayana\% Rehd.. Though analysis of variance (ANOVA) possesses a low resolution in mapping QTLs, it is an effective method for detecting QTLs. Single\|factor analysis of variance was conducted for each locus\|trait combination to identify QTLs. The results revealed that 10,10,4,9 and 12 markers were significantly associated with leaf length, leaf width, ratio of length to width of leaf, petiole length and leaf area respectively, which accounted for 66.23%, 61.82%, 32.86%, 59.67% and 81.79% of the total phenotypic variation respectively. Two\|factor analysis of variance showed that 4,2,1,5 and 7 significant interactions among these markers were associated with leaf length, leaf width, ratio of length to width of leaf, petiole length and leaf area respectively. Most of marker loci associated with these quantitative traits were on the 4th,12th,15th and 17th line of linkage groups.