文献类型：期刊文献

英文题名：Extraction and amplification of DNA from aged and archaeological Populus euphratica wood for species identification

作者：Jiao, Lichao[2] Liu, Xiaoli[3] Jiang, Xiaomei[2] Yin, Yafang[1]

第一作者：焦立超

通信作者：Yin, Yafang|[a00051fef300b63a7779c]殷亚方;[a00059471d20919e0b168]殷亚方;

机构：[1] State Key Laboratory of Tree Genetics and Breeding, Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing, 100091, China; [2] Wood Anatomy and Utilization Department, Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing, 100091, China; [3] Beijing Museum of Natural History, Beijing, 100050, China

年份：2015

卷号：69

期号：8

起止页码：925-931

外文期刊名：Holzforschung

收录：EI(收录号：20154001336929);Scopus(收录号：2-s2.0-84942774436)

语种：英文

外文关键词：Wood - Extraction - Polymerase chain reaction

摘要：The wood samples of Populus euphratica Oliv. (Salicaceae) are common archaeological plant remains in the hot and arid regions of western China. However, it is difficult to identify P. euphratica wood based on traditional wood anatomical methods alone. DNA barcoding might provide a higher security for species identification. In this study, aged wood specimens stored for approximately 30, 60, and 80 years and archaeological wood up to 3600 years old were in focus to explore the potential of DNA extraction and PCR amplification for different-sized fragments, ranging between 100 and 800 bp, taken from wood stored for different periods. The results indicated that DNA fragments of more than 100 bp could be successfully retrieved from a wood specimen stored for about 80 years based on a modified Qiagen kit protocol. However, it was impossible to obtain DNA segments from the 3600-year-old wood according to the current extraction protocol. Moreover, it was deduced that two-stage PCR amplification could play a significant role in the analysis of DNA retrieved from aged wood materials. With the aid of phylogenetic analysis, based on the short DNA barcode rbcL-2 of 202 bp in length, it was possible to differentiate P. euphratica from the other species of the Populus genus. ? 2015 by De Gruyter.