文献类型：期刊文献

英文题名：PmHs1^pro-1 monitors Bsursaphelenchus xylophilus infection and activates defensive response in resistant Pinus massoniana

作者：Xie, Yini[1,2] Liu, Bin[1,2] Zhou, Zhichun[1,2] Gao, Kai[1,2] Yin, Hengfu[1] Zhao, Yunxiao[1] Liu, Qinghua[1,2,3]

第一作者：Xie, Yini

通信作者：Liu, QH[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[2]Zhejiang Prov Key Lab Tree Breeding, Hangzhou, Zhejiang, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China

年份：2024

卷号：47

期号：11

起止页码：4369-4382

外文期刊名：PLANT CELL AND ENVIRONMENT

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001265001300001)】；

基金：National Key Research and Development Program of China, Grant/Award Number:2022YFD2200200; STI 2030-Major Projects, Grant/Award Number:2022ZD04016; Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding, Grant/Award Number:2021C02070-5-2; Forestry Science and Technology Innovation Special Project of Jiangxi Forestry Bureau, Grant/Award Number: 2021.No.13

语种：英文

外文关键词：defence mechanism; pine wood nematode; plant immune responses; R genes; ROS burst

摘要：Plant resistance (R) genes play a crucial role in the detection of effector proteins secreted by pathogens, either directly or indirectly, as well as in the subsequent activation of downstream defence mechanisms. However, little is known about how R genes regulate the defence responses of conifers, particularly Pinus massoniana, against the destructive pine wood nematode (PWN; Bursaphelenchus xylophilus). Here, we isolated and characterised PmHs1(pro-1), a nematode-resistance gene of P. massoniana, using bioinformatics, molecular biology, histochemistry and transgenesis. Tissue-specific expressional pattern and localisation of PmHs1(pro-1) suggested that it was a crucial positive regulator in response to PWN attack in resistant P. massoniana. Meanwhile, overexpression of PmHs1(pro-1) was found to activate reactive oxygen species (ROS) metabolism-related enzymes and the expressional level of their key genes, including superoxide dismutase, peroxidase and catalase. In addition, we showed that PmHs1(pro-1) directly recognised the effector protein BxSCD1of PWN, and induced the ROS burst responding to PWN invasion in resistant P. massoniana. Our findings illustrated the molecular framework of R genes directly recognising the effector protein of pathology in pine, which offered a novel insight into the plant-pathogen arms race.