文献类型：期刊文献

英文题名：Ectopic expression of a Catalpa bungei (Bignoniaceae) PISTILLATA homologue rescues the petal and stamen identities in Arabidopsis pi-1 mutant

作者：Jing, Danlong[1] Xia, Yan[1] Chen, Faju[2] Wang, Zhi[1] Zhang, Shougong[1] Wang, Junhui[1]

第一作者：Jing, Danlong

通信作者：Wang, JH[1]

机构：[1]Chinese Acad Forestry, State Key Lab Forest Genet & Tree Breeding, Key Lab Tree Breeding & Cultivat, Res Inst Forestry,State Forestry Adm, Beijing 100091, Peoples R China;[2]China Three Gorges Univ, Biotechnol Res Ctr, Yichang City 443002, Hubei Province, Peoples R China

年份：2015

卷号：231

起止页码：40-51

外文期刊名：PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000348261200005)】；

基金：This work was financially supported by the 12th Five-Year Plan of National Science and Technology Support of China (No. 2012BAD21B03). The experimental materials were provided by the National Catalpa bungei Germplasm Bank.

语种：英文

外文关键词：Catalpa bungei; B-class MADS box gene; PISTILLATA; Ectopic expression; Flower development; Functional conservation

摘要：PISTILLATA (PI) plays crucial roles in Arabidopsis flower development by specifying petal and stamen identities. To investigate the molecular mechanisms underlying organ development of woody angiosperm in Catalpa, we isolated and identified a PI homologue, referred to as CabuPI (C. bungei PISTILLATA), from two genetically cognate C bungei (Bignoniaceae) bearing single and double flowers. Sequence and phylogenetic analyses revealed that the gene is closest related to the eudicot PI homologues. Moreover, a highly conserved PI-motif is found in the C-terminal regions of CabuPI. Semi-quantitative and quantitative real time PCR analyses showed that the expression of CabuPI was restricted to petals and stamens. However, CabuPI expression in the petals and stamens persisted throughout all floral development stages, but the expression levels were different. In 35S::CabuPI transgenic homozygous pi-1 mutant Arabidopsis, the second and the third whorl floral organs produced normal petals and a different number of stamens, respectively. Furthermore, ectopic expression of the CabuPI in transgenic wild-type or heterozygote pi-1 mutant Arabidopsis caused the first whorl sepal partially converted into a petal-like structure. These results clearly reveal the functional conservation of PI homologues between C. bungei and Arabidopsis. (C) 2014 Elsevier Ireland Ltd. All rights reserved.