文献类型：期刊文献

英文题名：Temporal and Spatial Pattern of Indole-3-acetic Acid Occurrence during Walnut Pistillate Flower Bud Differentiation as Revealed by Immunohistochemistry

作者：Gao, Ying[1] Liu, Hao[1] Dong, Ningguang[1] Pei, Dong[1]

通信作者：Pei, D[1]|[a0005c81e28dc32effca6]裴东;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2012

卷号：137

期号：5

起止页码：283-289

外文期刊名：JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE

收录：;Scopus(收录号：2-s2.0-84866239993);WOS:【SCI-EXPANDED(收录号:WOS:000309024600002)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 31171933).

语种：英文

外文关键词：Juglans regia; Inflorescence; colloidal gold; immunohistochemical; auxin

摘要：We used anti-indole-3-acetic acid (IAA) monoclonal antibodies to monitor the temporal and spatial pattern of IAA during pistillate flower bud differentiation in the walnut (Juglans regia) cultivar Liaoning I. Based on morphological changes, the process of pistillate flower bud differentiation was divided into five stages. The flower induction stage, which includes the early phase, midphase, and late phase, persisted from 25 Apr. to the end of May. The pedicel differentiation stage began on 5 June. The bract primordium stage began on 25 June and persisted through mid-March of the next year. Both the perianth and pistil differentiation stages persisted for nearly 2 weeks. During the floral induction period, little IAA was present in the shoot apical meristem (SAM); hence, the SAM may not always be a site of IAA production. IAA was obviously concentrated in cells of the first several layers of the SAM during pedicel primordium formation. High levels of IAA were also noted in the phyllome, young leaf tips, and vascular bundle of leaves and gemmae. This direct evidence indicates that no close relationship exists between IAA and physiological differentiation; instead, IAA may strongly affect morphogenesis. These findings comprise a first step toward elucidating the walnut flowering mechanism.