文献类型：期刊文献

中文题名：空竹悬浮细胞系的建立

英文题名：ESTABLISHMENT OF SUSPENSION CELL LINE OF Cephalostachyum fuchsianum

作者：姚娜[1] 岳晋军[2] 顾小平[2] 杨凯[3] 李潞滨[1]

第一作者：姚娜

机构：[1]中国林业科学研究院林业研究所/国家林业局林木培育重点实验室;[2]中国林业科学研究院亚热带林业研究所;[3]北京农学院/农业应用新技术北京市重点实验室

年份：2011

卷号：25

期号：1

起止页码：43-47

中文期刊名：核农学报

外文期刊名：Journal of Nuclear Agricultural Sciences

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：浙江省重大科技专项重点项目(No.2009C12097);十一五科技支撑项目(2006BAD19B08);中国林业科学研究院亚热带林业研究所基金项目(No.RISF6908);林业公益性行业科研专项(No.200704001)

语种：中文

中文关键词：空竹;悬浮细胞;细胞培养

外文关键词：Cephalostachyum fuchsianum; suspension cells; cell culture

分类号：Q943.1

摘要：竹子悬浮细胞培养比较困难,本研究首次获得空竹悬浮细胞。以空竹种胚为材料诱导愈伤组织,建立稳定的悬浮细胞培养体系;通过固体培养法获得悬浮细胞来源的愈伤组织,可为竹类悬浮细胞系遗传转化或诱变后单克隆的筛选提供材料。空竹种胚在3/4MS+500mg/L脯氨酸+500mg/L谷氨酰胺+300mg/L胰蛋白胨+3.0mg/L 2,4-D+1.5mg/L KT+30g/L蔗糖+5g/L琼脂培养基上可脱分化出愈伤组织;愈伤组织在MS+500mg/L脯氨酸+500mg/L谷氨酰胺+300mg/L胰蛋白胨+1.5mg/L 2,4-D+30g/L蔗糖液体培养基中振荡培养3代后可建立稳定的悬浮细胞系,最佳继代周期为9d。悬浮细胞在固体培养基中培养25d后可增殖为愈伤组织,最佳接种密度为20g/L。
Cell suspension culture of bamboo is difficult.The suspension cell line of Cephalostachyum fuchsianum was established for the first time in this study.Seed embryos of Cephalostachyum fuchsianum were used to induce calli.The calli were dedifferentiated from the embyos on the medium of 3/4MS ＋ 500mg/L proline ＋ 500mg/L glutamine ＋ 300mg/L tryptone ＋ 3.0mg/L 2,4-D ＋ 1.5mg/L KT ＋ 30g/L sucrose ＋ 5g/L agar.A fine suspension cell line could be established by inoculating the calli in the medium of MS ＋ 500mg/L proline ＋ 500mg/L glutamine ＋ 300mg/L tryptone ＋ 1.5mg/L 2,4-D ＋ 30g/L sucrose after 3 times shaking subcultures.The subculture cycle was 9d.The calli could be acquired from suspension cell line after the cells being cultured for 25d in the solid medium,and the appropriate inoculation density was 20g/L.