文献类型：期刊文献

中文题名：云南金沙江干热河谷土壤微生物群落结构特征及影响因素

英文题名：Soil microbial community structure and influencing factors in dry?hot valley region of Jinsha River,Yunnan Province

作者：刘顺[1,2] 许格希[1,2] 陈健[1,2] 陈淼[1,2] 史作民[1,2,3] 周庆宏[4] 蒋冬梅[4]

第一作者：刘顺

机构：[1]中国林业科学研究院森林生态环境与自然保护研究所,国家林业和草原局森林生态环境重点实验室,北京100091;[2]四川米亚罗森林生态系统国家定位观测研究站,理县623100;[3]南京林业大学南方现代林业协同创新中心,南京210037;[4]楚雄彝族自治州林业和草原科学研究所,楚雄675005

年份：2024

卷号：44

期号：16

起止页码：7172-7182

中文期刊名：生态学报

外文期刊名：Acta Ecologica Sinica

收录：CSTPCD;;国家哲学社会科学学术期刊数据库;Scopus;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：云南省科技人才与专家计划“史作民专家工作站”项目(202305AF150100);中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2021ZA002-2)。

语种：中文

中文关键词：磷脂脂肪酸;河谷型萨王纳植被;草本生物量;金沙江

外文关键词：phospholipid fatty acid;Savanna of valley type;herb biomass;Jinsha River

分类号：S154.3

摘要：金沙江干热河谷区是长江上游重要的生态屏障和典型生态脆弱区,对金沙江干热河谷土壤微生物群落结构变异特征及其驱动因素的了解仍不清楚。沿着金沙江选择5个地点的典型干热河谷植被,采用磷脂脂肪酸法研究了土壤微生物生物量和群落结构的空间变异,并探讨了气候、植被因子和土壤性质等对土壤微生物群落结构的影响。结果表明:不同研究点土壤总微生物生物量介于4.30—13.20 nmol/g,以细菌为主,其中革兰氏阳性菌(G+)占26.22%—33.65%,革兰氏阴性菌(G-)占18.35%—23.67%;真菌占9.02%—15.13%。不同研究点土壤微生物生物量、真菌/细菌和G+/G-差异显著,并且非度量多维尺度分析形成不同聚类,表明土壤微生物群落结构在研究点间发生了变化。方差分解结果揭示了土壤微生物群落结构的变异主要受土壤因子的驱动,其次是植被和气候因素。草本生物量、土壤硝态氮、全碳、pH和含水量对土壤微生物群落结构具有显著影响。气候因素中年均降雨量对微生物生物量呈负影响效应。揭示了不同研究点通过土壤性质驱动了土壤微生物群落结构的变异,并发现了草本生物量在其中的重要作用,对干热河谷区植被恢复具有重要的意义。
Soil microbial communities play an important regulatory role in soil structural formation,nutrient cycling,and plant growth in terrestrial ecosystems,which are of great significance to the reestablishment of functions in degraded ecosystems.The dry?hot valley region of Jinsha River is an important ecological barrier in the upper reaches of the Yangtze River,and a typical ecologically fragile area.However,the understanding of variations in soil microbial community structure and driving factors in this region remains unclear.Here,the variations in soil microbial biomass and community structure of typical dry?hot valley across five sites along the Jinsha River basin were investigated using phospholipid fatty acid(PLFA)analysis.Further,the effects of climatic,vegetation and edaphic factors on soil microbial community structure were explored.The results showed that total soil microbial biomass ranged from 4.30 nmol/g to 13.20 nmol/g across the five sites.Bacterial biomass accounted for the largest proportion of total microbial biomass,with gram?positive bacterial(G+)and gram?negative bacterial(G-)biomass accounting for 26.22%—33.65%and 18.35%—23.67%,respectively.The proportion of fungal biomass in total microbial biomass was between 9.02%and 15.13%.These sites had significant difference in microbial biomass of total and specific functional group microorganisms,fungi/bacteria,G+/G-,cyclopropane fatty acids/monoenoic precursors,normal saturated fatty acids/monounsaturated fatty acids.Moreover,their soil microbial community structure formed different clusters based on the non?metric multidimensional scale(NMDS)analysis,suggesting significant differences among the sites.The results of variation partitioning analysis(VPA)revealed that the variation of soil microbial community structure was mainly driven by edaphic factors,followed by vegetation and climatic factors with the pure effects being 37%,16%and 2%,respectively.Specifically,herb biomass(P=0.002),soil NO_(3)^(-)(P=0.002),soil total carbon(P=0.003),pH(P=0.013)and soil water content(P=0.029)had significant effects on soil microbial community structure.Generally,herb biomass,soil total carbon and O_(3)^(-)content had positive effects,while soil water content and pH had negative effects on the PLFAs contents of total microbes and specific functional groups.In addition,the microbial PLFAs of various functional groups were also affected by the ratios of total carbon to total nitrogen and the ratio of total nitrogen to total phosphorus.The mean annual precipitation of climate factors had a negative impact on soil microbial biomass.Overall,the results revealed site?induced shifts in edaphic factors drove the variations in soil microbial community structure,and found the important role of herb biomass,which had vital implications for vegetation restoration in dry?hot valley region.