文献类型：期刊文献

英文题名：Expression of the SPL-like gene LaSPL9 in Japanese larch (Larix leptolepis) is regulated by miR156 during somatic embryogenesis

作者：Zhang, Li-feng[1] Fan, Yan-ru[1] Lan, Qian[2] Qi, Li-wang[1] Han, Su-ying[3]

第一作者：张立峰

通信作者：Zhang, LF[1];Han, SY[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm,Res Inst Forestry, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry Policy & Informat, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing 100091, Peoples R China

年份：2021

卷号：35

期号：5

起止页码：1727-1737

外文期刊名：TREES-STRUCTURE AND FUNCTION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000607973500001)】；

基金：This work was supported by the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2019SY011), the National Natural Science Foundation of China (31600544 and 31330017), and the National Transgenic Major Program of China (2018ZX08020-003). We thank Liwen Bianji, Edanz Editing China (www.liwenbianji.cn/ac) for editing the English text of a draft of this manuscript.

语种：英文

外文关键词：Larix leptolepis; LaSPL9; miR156; Somatic embryogenesis; GA; SA

摘要：A full-length cDNA sequence of a SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL) gene was isolated from Japanese larch (Larix leptolepis) and designated as LaSPL9. The cDNA comprised 2735 bp, including a 1779-bp open reading frame encoding a protein (592 amino acids) with conserved SBP domains. The LaSPL9 promoter region included several gibberellin (GA)- and salicylic acid (SA)-inducible elements. Further analyses revealed that LaSPL9 mRNA is targeted by miR156. An examination of the LaSPL9-GFP fusion protein in tobacco leaf epidermal cells indicated that LaSPL9 is a nuclear protein. Additionally, LaSPL9 expression was induced by GA and the miR156b precursor (premiR156b) transcript accumulation was inhibited by SA. Moreover, abscisic acid (ABA) was identified as the main factor down-regulating LaSPL9 expression during the early stage of somatic embryogenesis (SE), which can induce premiR156b expression. Furthermore, LaSPL9 transcription peaked at 10 days, implying it might encode an important regulator of early embryonic pattern formation. The LaSPL9 transcript level subsequently decreased gradually and was lowest at 42 days, which is consistent with the gradual increase in miR156 expression during SE. The miR156-LaSPL9 module might regulate SE in L. leptolepis by responding to ABA. Overall, our results provide evidence that miR156-LaSPL9 is important for the crosstalk between GA and SA pathways during SE.