文献类型：期刊文献

中文题名：基于表型的灰楸核心种质构建

英文题名：Construction of Core Collection of Catalpa fargesii Bur.Based on Phenotype

作者：于晓池[1,2] 李凤[3] 欧阳[3] 张鹏[2] 郭小龙[4] 肖遥[1] 赵秋玲[4] 杨桂娟[1,5] 王军辉[1,5] 麻文俊[1,5]

第一作者：于晓池

机构：[1]林木遗传育种国家重点实验室,国家林业和草原局林木培育重点实验室,中国林业科学研究院林业研究所,北京100091;[2]东北林业大学,黑龙江哈尔滨150040;[3]西南林业大学,甘肃天水741002;[4]小陇山林业科学研究所,云南昆明650000;[5]国家林业和草原局楸树国家创新联盟,北京100091

年份：2021

卷号：34

期号：6

起止页码：38-45

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家重点研发计划课题“楸树良种选育与高效培育技术研究”(2017YFD0600604)。

语种：中文

中文关键词：灰楸;表型性状;遗传变异;遗传多样性;核心种质构建

外文关键词：Catalpa fargesii Bur.;phenotypic traits;genetic variation;genetic diversity;core germplasm

分类号：S772.8

摘要：[目的]为得出较为可靠的灰楸初级核心种质群体,以加强灰楸种质选育、开发利用和分子遗传学研究,解决其种质资源保存成本较高问题,促进灰楸种质资源的鉴定和有效利用。[方法]本研究以来自甘肃省、陕西省、山西省和河南省的200个11年生灰楸无性系的叶部性状,13年生的生长性状和材性性状为依据,采用5种距离计算方法、6种系统聚类方法、3种取样方法和5种取样比例构建灰楸初级核心种质资源,并进行评价。[结果]采用欧式距离和最短距离系统聚类法,以15%的取样比例进行偏离度取样构建的包括30个无性系的灰楸初级核心种质,均值差异百分率为10.00%、方差差异百分数为40.00%、极差符合率为91.95%、变异系数变化率为136.96%,最能代表原有的种质群体;核心种质的构建提高了叶长、叶宽、叶长宽比、叶柄长、胸径、冠幅和弹性模量高于均值的种质份数占比,降低了Pilodyn值高于均值的种质份数占比;陕西省、山西省和河南省的核心种质份数占比均高于原始种质。[结论]灰楸种质各性状均存在丰富的遗传变异,30份核心种质不仅还原了原种质的均值、极差和变异程度,还提高了种质的变异系数,略提高了核心种质的生长和材性水平,为促进种质资源的鉴定和有效利用奠定了基础。
[Objective]To obtain a more reliable primary core germplasm group of Catalpa fargesii,to strengthen the breeding,development and utilization and molecular genetics research,to reduce the conservation cost of germplasm resources,and to promote the identification and effective utilization of C.fargesii germplasm resources.[Method]Based on the leaf traits,the growth traits and the wood traits of 200 C.fargesii clones from Gansu,Shaanxi,Shanxi and He’nan provinces,five genetic distance calculation methods,six clustering methods,three sampling methods and five sampling ratios were used to construct the primary core germplasm resources,and then evaluated them.[Result]Using the Euclidean distance and the Single system clustering method,the deviation sampling with 15%sampling proportion was adopt to construct the primary core collection of 30 clones,the mean difference percentage was 10%,the variance difference percentage was 40%,the coincidence rate of range was91.95%,and the changeable rate of coefficient of variation was 136.96%,which could best represent the original germplasm group.The construction of core collection increased the percentage of germplasm with leaf length,leaf width,leaf length width ratio,petiole length,diameter at breast height,crown width and elastic modulus higher than the average,and reduced the percentage of germplasm with Pilodyn value higher than the average.The percentage of core collection in Shaanxi,Shanxi and He’nan provinces were higher than that of the original collection.[Conclusion]This research showed that there are abundant genetic variation in each trait of C.fargesii clones.The30 core germplasms not only retain the mean,range and degree of variation of the original germplasms,but also increase the variationcoefficient of the germplasms,and slightly increase the growth and the material quality level of the germplasms.This research laid a foundation for promoting the identification and effective utilization of germplasm resources.