文献类型：期刊文献

中文题名：黄金树花器官特征决定的CaspAG基因克隆和表达分析

英文题名：Cloning and Expression Analysis of an AG AMOUS-Like Gene( Casp AG) from Catalpa speciosa

作者：夏燕[1] 景丹龙[1] 王军辉[1]

第一作者：夏燕

机构：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,国家林木种质资源平台,北京100091

年份：2014

卷号：50

期号：10

起止页码：1523-1528

中文期刊名：植物生理学报

外文期刊名：Plant Physiology Journal

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金："十二五"国家科技支撑项目(2012BAD21B03)

语种：中文

中文关键词：黄金树;花发育;AGAMOUS同源基因;实时荧光定量PCR

外文关键词：Catalpa speciosa; flower development; AGAMOUS-like gene; qRT-PCR

分类号：S562

摘要：以黄金树的花芽为材料,采用同源基因克隆技术,获得黄金树花器官特征决定的AG同源基因,将其命名为CaspAG,其开放阅读框(ORF)为738 bp,编码245个氨基酸。分子系统发生和蛋白序列比对分析表明:CaspAG是拟南芥的AG同源蛋白,被归为eu AG进化分支,其MADS区有57个氨基酸,I区有32个氨基酸,K区有83个氨基酸,C区有55个氨基酸,其中C末端的转录激活区含有两个保守的基序:AGI和AGII基序。半定量RT-PCR分析表明,在花发育过程中,CaspAG基因仅在雄蕊和雌蕊中表达,而在茎、叶片、萼片和花瓣中几乎不表达。实时荧光定量PCR分析结果表明,CaspAG基因在雌雄蕊原基分化期至雌雄蕊成熟期均有表达,在雌雄蕊发育成熟期表达量达到最高;且在雄蕊的表达高峰的时间明显早于雌蕊,这与雌、雄蕊形态成熟的时间基本吻合。
A MADS-box gene CaspAG involved in flower development was isolated from the flower bud of Catalpa speciosa via homology cloning technique. The open reading flame （ORF） of CaspAG was 738 bp, which encoded 245 amino acid. Protein sequence alignment and molecular phylogeny analysis suggested that CaspAG was an AG homology in Arabidopsis, grouped with euAG clade. Conceptual translation revealed that the MADS domain contained 57 amino acid, the I domain contained 32 amino acid, the K domain contained 83 amino acid, the C domain contained 55 amino acid. Moreover, two highly conserved motifs specific to C pro- teins, AG motifs I and II, were found in the C-terminal regions of the CaspAG protein. Semi-quantitative RT- PCR analysis showed CaspA G transcriptional activity mainly concentrated on stamens and carpels while no-ex- pression in plant stem, leaves, sepals and petals during different stages of flower development. The qRT-PCR analysis showed that the expression of CaspAG was detected at the primordium to mature stages of stamen and pistil during morphological differentiation of organs. The expression was highest at the mature stages of stamen and pistil during morphological differentiation of organs. The date of expression peak appeared in stamens ear- lier than that in pistils, and the time of expression was the same with morphological differentiation of the sta- men and pistil.