文献类型：期刊文献

英文题名：Hierarchical Transcription Factor and Chromatin Binding Network for Wood Formation in Populus trichocarpa

作者：Chen, Hao[1,2,3] Wang, Jack P.[1,2] Liu, Huizi[1] Li, Huiyu[1] Lin, Ying-Chung Jimmy[1,4,5] Shi, Rui[2] Yang, Chenmin[2] Gao, Jinghui[1] Zhou, Chenguang[1] Li, Quanzi[6] Sederoff, Ronald R.[2] Li, Wei[1,2] Chiang, Vincent L.[1,2,7]

第一作者：Chen, Hao

通信作者：Chiang, VL[1];Chiang, VL[2];Chiang, VL[3]

机构：[1]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Heilongjiang, Peoples R China;[2]North Carolina State Univ, Dept Forestry & Environm Resources, Forest Biotechnol Grp, Raleigh, NC 27695 USA;[3]North Carolina State Univ, Dept Genet, Raleigh, NC 27695 USA;[4]Natl Taiwan Univ, Coll Life Sci, Dept Life Sci, Taipei 10617, Taiwan;[5]Natl Taiwan Univ, Coll Life Sci, Inst Plant Biol, Taipei 10617, Taiwan;[6]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[7]North Carolina State Univ, Dept Forest Biomat, Raleigh, NC 27695 USA

年份：2019

卷号：31

期号：3

起止页码：602-626

外文期刊名：PLANT CELL

收录：;Scopus(收录号：2-s2.0-85064988328);WOS:【SCI-EXPANDED(收录号:WOS:000464927400007)】；

基金：This work was supported by the National Natural Science Foundation of China (grant nos. 31430093 and 31522014); China Postdoctoral Science Foundation (grant 2015M581410); Heilongjiang Postdoctoral Financial Assistance (grant no. LBH-Z15008); and the Innovation Project of State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University) grant no. A01. We also acknowledge the financial support from the 1000-talents Plan for young researchers from China and Longjiang Young Scholar Program of Heilongjiang Provincial Government (to W.L.), the U.S. Department of Energy (Biological and Environmental Research) (grant no. DE-SC000691 to V.L.C.), Taiwan Ministry of Science and Technology MOST (grant nos. 106-2311-B-002-001-MY2 and 107-2636-B-002-003 to Y.-C.J.L.), the Fundamental Research Funds for the Central Universities of China (grant no. 2572018CL01), and the 111 Project of the Fundamental Research Funds (grant no. B16010).

语种：英文

摘要：Wood remains the world's most abundant and renewable resource for timber and pulp and is an alternative to fossil fuels. Understanding the molecular regulation of wood formation can advance the engineering of wood for more efficient material and energy productions. We integrated a black cottonwood (Populus trichocarpa) wood-forming cell system with quantitative transcriptomics and chromatin binding assays to construct a transcriptional regulatory network (TRN) directed by a key transcription factor (TF), PtrSND1-B1 (secondary wall-associated NAC-domain protein). The network consists of four layers of TF-target gene interactions with quantitative regulatory effects, describing the specificity of how the regulation is transduced through these interactions to activate cell wall genes (effector genes) for wood formation. PtrSND1-B1 directs 57 TF-DNA interactions through 17 TFs transregulating 27 effector genes. Of the 57 interactions, 55 are novel. We tested 42 of these 57 interactions in 30 genotypes of transgenic P. trichocarpa and verified that similar to 90% of the tested interactions function in vivo. The TRN reveals common transregulatory targets for distinct TFs, leading to the discovery of nine TF protein complexes (dimers and trimers) implicated in regulating the biosynthesis of specific types of lignin. Our work suggests that wood formation may involve regulatory homeostasis determined by combinations of TF-DNA and TF-TF (protein-protein) regulations.