文献类型：期刊文献

英文题名：Physiological response and molecular mechanism of Quercus variabilis under cadmium stress

作者：Tan, Cancan[1,2] Nie, Wen[1] Liu, Yifu[1] Wang, Ya[3] Yuan, Yanchao[1] Liu, Jianfeng[3] Chang, Ermei[3] Xiao, Wenfa[1] Jia, Zirui[2,3]

第一作者：Tan, Cancan

通信作者：Jia, ZR[1]

机构：[1]Chinese Acad Forestry, Natl Forestry & Grassland Adm, Ecol & Nat Conservat Inst, Key Lab Forest Ecol & Environm, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2024

卷号：211

外文期刊名：PLANT PHYSIOLOGY AND BIOCHEMISTRY

收录：;Scopus(收录号：2-s2.0-85193027459);WOS:【SCI-EXPANDED(收录号:WOS:001240699200001)】；

基金：This work was supported by the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2022QC003- 02) .

语种：英文

外文关键词：Quercus variabilis; Cd stress; NMT; Transcriptome; DEGs; WGCNA

摘要：Heavy metal pollution is a global environmental problem, and Quercus variabilis has a stronger tolerance to Cd stress than do other species. We aimed to explore the physiological response and molecular mechanisms of Q. variabilis to Cd stress. In this study, the antioxidant enzyme activities of leaves were determined, while the photosynthetic parameters of leaves were measured using Handy PEA, and ion fluxes and DEGs in the roots were investigated using noninvasive microtest technology (NMT) and RNA sequencing techniques, respectively. Cd stress at different concentrations and for different durations affected the uptake patterns of Cd2+ and H+ by Q. variabilis and affected the photosynthetic efficiency of leaves. Moreover, there was a positive relationship between antioxidant enzyme (CAT and POD) activity and Cd concentration. Transcriptome analysis revealed that many genes, including genes related to the cell wall, glutathione metabolism, ion uptake and transport, were significantly upregulated in response to cadmium stress in Q. variabilis roots. WGCNA showed that these DEGs could be divided into eight modules. The turquoise and blue modules exhibited the strongest correlations, and the most significantly enriched pathways were the phytohormone signaling pathway and the phenylpropanoid biosynthesis pathway, respectively. These findings suggest that Q. variabilis can bolster plant tolerance by modulating signal transduction and increasing the synthesis of compounds, such as lignin, under Cd stress. In summary, Q. variabilis can adapt to Cd stress by increasing the activity of antioxidant enzymes, and regulating the fluxes of Cd2+ and H+ ions and the expression of Cd stress-related genes.