文献类型：期刊文献

英文题名：Effects of exogenous GABA on gene expression of Caragana intermedia roots under NaCl stress: regulatory roles for H2O2 and ethylene production

作者：Shi, Sheng-Qing[1,2] Shi, Zheng[] Jiang, Ze-Ping[1,2] Qi, Li-Wang[1,2] Sun, Xiao-Mei[1,2] Li, Chun-Xiu[3] Liu, Jian-Feng[1,2] Xiao, Wen-Fa[] Zhang, Shou-Gong[1,2]

第一作者：史胜青

通信作者：Zhang, SG[1]

机构：[1]Chinese Acad Forestry, Inst Forestry Res, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Inst Forest & Ecol Protect, Beijing 100091, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biocatalysis & Bioproc, Shanghai 200237, Peoples R China

年份：2010

卷号：33

期号：2

起止页码：149-162

外文期刊名：PLANT CELL AND ENVIRONMENT

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000273551100002)】；

基金：We thank Professor Rong-fu Gao for suggestion of this study, Dr. Hong-quan Wang for the help with statistical analysis, Dr. Hong-en Jiang for the help with proofreading and Edanz editing for further editing the manuscript. This work was supported by National S&T Support Program for 11th Five-Year Plan (2006BAD01A1606, 2006BAD03A0104), China Postdoctoral Science Foundation Funded Project (20070410578), National '973' Project (2009CB119107) and Forestry S&R Special Project of SFA (200804001). Partial work was completed at Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, China.

语种：英文

外文关键词：suppression subtractive hybridization; gene expression; signal transduction; reactive oxygen species; nitrogen sources; proline

摘要：gamma-aminobutyric acid (GABA) is a four-carbon non-protein amino acid presented in a wide range of organisms. In this study, a suppression subtractive hybridization (SSH) library was constructed using roots of a legume shrub, Caragana intermedia, with the combined treatment of 300 mM NaCl and 300 mM NaCl + 10 mM GABA. We obtained 224 GABA-regulated unique expressed sequence tags (ESTs) including signal transduction, transcriptional regulation, hormone biosynthesis, reactive oxygen species (ROS) and polyamine metabolism, etc. The key H2O2-generated genes, NADPH oxidase (CaGR60), peroxidase (CaGR61) and amine oxidase (CaGR62), were regulated at the mRNA level by 10 mM GABA, which clearly inhibited H2O2 accumulation brought about by NaCl stress in roots and leaves with the observation of 3,3'-diaminobenzidine (DAB) staining. Similarly, 10 mM GABA also regulated the expression of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) genes (CaGR30 and CaGR31) and ethylene production in NaCl-treated roots. Surprisingly, these H2O2-generated genes were enhanced at the mRNA level by a lower concentration of GABA, at 0.25 mM, but not other alternative nitrogen sources, and endogenous GABA accumulated largely just by the application of GABA at either concentration. Our results further proved that GABA, as a signal molecule, participates in regulating the expression of genes in plants under salt stress.