文献类型：期刊文献

中文题名：三种药用兰科植物内生微生物多样性与功能分析

英文题名：Diversity and functions of endophytic microorganisms in three medicinal orchids

作者：姚娜[1] 王涛[2] 王雁[1] 孙嫚潞[1] 郑宝强[1] 曹晓璐[1]

第一作者：姚娜

机构：[1]中国林业科学研究院林业研究所、国家林业和草原局林木培育重点实验室、林木遗传育种国家重点实验室,北京100091;[2]国家植物园(北园)植物迁地保护国家林业和草原局重点实验室,北京100093

年份：2025

卷号：52

期号：3

起止页码：1166-1179

中文期刊名：微生物学通报

外文期刊名：Microbiology China

收录：;北大核心:【北大核心2023】；

基金：中央级公益性科研院所基本科研业务费专项基金(CAFYBB2019ZA001);国家自然科学基金(32101497)。

语种：中文

中文关键词：卵叶贝母兰;竹叶兰;手参;深色有格内生真菌

外文关键词：Coelogyne occultata;Arundina graminifolia;Gymnadenia conopsea;dark septate endophyte

分类号：S567.1

摘要：【背景】兰科植物根部内生微生物在促进幼苗生长、提高植株环境适应性等方面发挥重要作用。【目的】通过对卵叶贝母兰(Coelogyne occultata)、竹叶兰(Arundina graminifolia)和手参(Gymnadenia conopsea)这3种药用兰科植物根部内生微生物进行多样性分析与菌株分离培养,解析不同兰科植物内生微生物群落结构,筛选功能性菌株。【方法】采用Illumina MiSeq高通量测序技术分析3种药用兰科植物根部内生微生物群落结构,采用微生物分离培养方法分离根部内生细菌和内生真菌,并进行功能菌株筛选。【结果】在属水平,卵叶贝母兰和竹叶兰的内生微生物群落聚为一支。卵叶贝母兰、竹叶兰和手参内生细菌优势属分别为分枝杆菌属(Mycobacterium,70.65%),伯克霍尔德氏菌属-卡瓦列罗菌属-副伯克霍尔德氏菌属(Burkholderia-Caballeronia-Paraburkholderia,26.35%)和戈登氏菌属(Gordonia,6.65%);内生真菌优势属分别为镰刀菌属(Fusarium,21.07%),未分类柔膜菌目(unclassified Helotiales,46.74%)和羊肚菌属(Morchella,91.21%)。Pseudomonas nunensis Coeb11、Rahnella sikkimica Coeb32和森林鲁氏菌(Rouxiella silvae)Coeb22同时具有溶磷、固氮和分泌吲哚乙酸(indole-3-acetic acid,IAA)的功能;真菌Cladophialophora chaetospira Aruf26可作为深色有格内生真菌定殖在铁皮石斛根细胞内部。【结论】本研究揭示了3种药用兰科植物根部内生微生物群落组成,筛选出功能菌株,为药用兰科植物资源保护、林下仿生栽培和功能微生物的开发与利用提供依据。
[Background]Endophytic microorganisms in the roots can promote the seedling growth and improve the environmental adaptability of orchids.[Objective]To reveal the structures of endophytic microbial communities in three medicinal orchids(Coelogyne occultata,Arundina graminifolia,and Gymnadenia conopsea)and screen out functional strains.[Methods]Illumina MiSeq high-throughput sequencing was employed to analyze the structures of endophytic microbial communities in the roots of the three orchids.Conventional methods were adopted to isolate the endophytic bacteria and fungi from the roots,and the functional microorganisms were screened.[Results]The endophytic microbial communities of C.occultata and A.graminifolia clustered into one group at the genus level.In C.occultata,A.graminifolia,and G.conopsea,the dominant endophytic bacterial genera were Mycobacterium(Coe:70.65%),Burkholderia-Caballeronia-Paraburkholderia(Aru:26.35%),and Gordonia(Gym:6.65%),respectively;the dominant endophytic fungal genera were Fusarium(Coe:21.07%),unclassified Helotiales(Aru:46.74%),and Morchella(Gym:91.21%),respectively.Bacterial isolates Pseudomonas nunensis Coeb11,Rahnella sikkimica Coeb32,and Rouxiella silvae Coeb22 had the functions of dissolving phosphorus,fixing nitrogen,and secreting indole-3-acetic acid.The hyphae of the fungal isolate Cladophilophora chaetospira Aruf26 could colonize the cells of Dendrobium officinale as the dark septate endophyte.[Conclusion]This study revealed the structures of endophytic microbial communities in the roots of three medicinal orchids and screened the functional microorganisms.The findings provide a scientific basis for the protection of medicinal orchids,biomimetic cultivation under forests,and development and utilization of functional microorganisms.