文献类型：期刊文献

中文题名：苜蓿霜霉病接种方法及染病植株体内酶活性

英文题名：Inoculation method of downy mildew of alfalfa and enzyme activity in infected plants

作者：于良斌[1,2] 张园园[1] 王丹阳[3] 崔进[1] 徐林波[1] 闫丽英[4] 王慧[1] 崔艳伟[1]

第一作者：于良斌

机构：[1]中国农业科学院草原研究所,内蒙古呼和浩特010010;[2]黑龙江省农业科学院植物保护研究所,黑龙江哈尔滨150086;[3]中国林业科学研究院沙漠林业实验中心,内蒙古磴口015200;[4]内蒙古鄂尔多斯市农牧业科学研究院,内蒙古鄂尔多斯017000

年份：2023

卷号：40

期号：4

起止页码：935-941

中文期刊名：草业科学

外文期刊名：Pratacultural Science

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：公益性行业(农业)科研专项课题(201303057);内蒙古自治区科技计划项目(2020GG0141)。

语种：中文

中文关键词：苜蓿霜霉病;紫花苜蓿;接种方法;过氧化氢酶;过氧化物酶;苯丙氨酸解氨酶;超氧化物歧化酶

外文关键词：Peronospora aestivalis;alfalfa;inoculation method;catalase;peroxidase;phenylalanine ammonia-lyase;superoxide dismutase

分类号：S435.4

摘要：苜蓿霜霉病(Peronospora aestivalis)是苜蓿上的重要病害之一,严重限制了苜蓿产业的发展。为了探明苜蓿霜霉菌侵染对苜蓿体内保护酶活性的影响及优化苜蓿霜霉菌的接种方法,本研究以紫花苜蓿‘中草3号’(Medicago sativa‘Zhongcao No.3’)为研究对象,进行苜蓿霜霉菌的接种试验,并研究不同染病程度下过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)的酶活性,评价接种苜蓿霜霉菌对苜蓿体内保护酶活性的影响。结果表明:4种接种方法中病枝插入法不能获得成功接种的病株,病叶摩擦法、培养箱内喷雾法和培养箱外喷雾法能够得到接种的病株,发病率分别为11.1%、88.9%和25.6%,由此可以看出培养箱内接种法的接种效果最佳。接菌对苜蓿体内酶活性影响显著,苜蓿霜霉菌轻度侵染会导致CAT活性显著增强(P<0.05),较健康苜蓿,苜蓿霜霉菌侵染能够显著增强POD和SOD的活性(P<0.05),但是重度染病情况下与健康苜蓿相比SOD活性差异不显著(P>0.05);PAL活性表现为随着病原菌的侵染程度加深显著下降(P<0.05)。综上表明病原菌侵染会导致苜蓿体内酶活性的变化,本研究可为优化苜蓿霜霉菌的接种方法及通过苜蓿体内酶活性变化筛选抗性品种提供理论参考。
Downy mildew(Peronospora aestivalis)is a common disease found on alfalfa and has severely curbed the development of the alfalfa industry.To explore the effect of downy mildew infection on the protective enzyme activities in alfalfa and optimize the inoculation method of downy mildew of alfalfa,this study took alfalfa‘Zhongcao No.3’as the research object,inoculated it with downy mildew,and investigated the enzyme activity determination of catalase(CAT),superoxide dismutase(SOD),peroxidase(POD),and phenylalanine ammonia-lyase(PAL)under different infection degrees.The results showed that,among the four inoculation methods,the diseased branch insertion method could not successfully inoculate diseased plants,and the diseased leaf friction,spray method in the incubator and outside the incubator could successfully inoculate diseased plants,with incidence rates of 11.1%,88.9%,and 25.6%,respectively.The spray method in the incubator was observed to have the best inoculation effect.The inoculation had a significant impact on the enzyme activity in alfalfa,the mild downy mildew infection of alfalfa could cause CAT,and the activity was significantly enhanced(P<0.05).Compared with healthy alfalfa,alfalfa infected with downy mildew could significantly enhance POD and SOD activity(P<0.05),however,there was no significant difference in SOD activity between severe infection and healthy alfalfa.Moreover,the PAL activity showed a significant decrease as the degree of pathogen infection deepened(P<0.05).This shows that the invasion of pathogenic bacteria can cause changes in the enzyme activity in alfalfa,and this study provides theoretical reference for the inoculation of downy mildew in alfalfa and the screening of resistant varieties by the changes in enzyme activities in alfalfa.