文献类型：期刊文献

中文题名：基于油楠(Sindora glabra)转录组测序的SSR分子标记的开发

英文题名：Development of SSR Molecular Markers Based on Transcriptome Sequencing of Sindora glabra

作者：何畅[1] 杨锦昌[1] 余纽[1] 刘振湘[1] 覃国铭[1] 陈朝黎[1]

第一作者：何畅

机构：[1]中国林业科学研究院热带林业研究所,热带林业研究国家林草局重点实验室,广州510520

年份：2020

卷号：18

期号：7

起止页码：2280-2289

中文期刊名：分子植物育种

外文期刊名：Molecular Plant Breeding

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金项目(31570656)资助。

语种：中文

中文关键词：油楠(Sindora;glabra);转录组;SSR;分子标记

外文关键词：Sindora glabra;Transcriptome;SSR;Molecular markers

分类号：S51

摘要：油楠(Sindora glabra)作为极具开发潜力的能源植物,在分子水平上的研究仅局限于ISSR分子标记。本研究利用Trinity软件对油楠转录组数据进行组装,共得到总长为48307806 bp的283998条Unigenes。利用MISA软件寻找到97443个含有SSR的重复基元。SSR位点中主要重复序列为单核苷酸和二核苷酸,分别占总SSR位点的67.68%和22.56%,其次是三核苷酸,占8.54%。利用Primer 3.0设计引物,并从中随机选择200对引物进行PCR扩增,其中有13对扩增出清晰条带,多态性高,重复性好。油楠SSR分子标记的开发对于研究其遗传多样性、重要性状基因定位及分子标记辅助选择育种等起到重要的推动作用。
Sindora glabra is an important potential energy plant.However,the research about S.glabra at molecular level is limited to development of the ISSR molecular markers.In this research,a total of 283998 unigenes were obtained with total length of 483078066 bp according to the assembly of transcriptome sequencing data in S.glabra by using Trinity software.There were 97443 repeat contigs containing SSR were found by MISA software.Mono-nucleotide and di-nucleotide were the dominant repetitive sequences in SSR loci,which accounted for67.68%and 22.56%,respectively,followed by the trinucleotide with 8.54%.In the primers designed by Primer3.0,200 pairs of primers were randomly selected for PCR amplification.Among them,13 pairs of primers could amplify with clear bands,high polymorphism and good repeatability.These SSR markers would be useful for the analysis of the genetic diversity,location of candidate trait genes and molecular marker-assisted breeding of Sindora glabra.