文献类型：期刊文献

中文题名：不同抗性杨树接种溃疡病菌后过氧化氢及其氧化酶的表达差异

英文题名：Difference in Expression of Hydrogen Peroxide and Oxidative Enzymes in Different Resistant Poplars Inoculated with Botryosphaeria dothidea

作者：马健[1] 刘振宇[2] 吕全[1] 梁军[1] 严东辉[1] 张星耀[1]

第一作者：马健

机构：[1]国家林业局森林生态学与森林保护学重点实验室(中国林业科学研究院森林生态环境与保护研究所),北京100091;[2]山东农业大学

年份：2012

卷号：40

期号：12

起止页码：118-122

中文期刊名：东北林业大学学报

外文期刊名：Journal of Northeast Forestry University

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：国家林业公益性行业科研专项(201204501);国家科技部基础性工作专项(2009FY210100)

语种：中文

中文关键词：杨树;溃疡病菌;过氧化氢;抗坏血酸过氧化物酶;过氧化物酶

外文关键词：Populus spp. ; Botryosphaeria dothidea; Hydrogen peroxide; APX; POD

分类号：S764

摘要：为探讨不同抗性杨树接种溃疡病菌(Botryosphaeria dothidea)后过氧化氢(H2O2)及相关酶与其抗性间的关系,对抗病毛白杨(Populus tomentosa)和感病北京杨(P.×beijingensis)接种溃疡病菌后H2O2摩尔质量浓度、APX和POD酶活性及基因表达进行了研究。结果发现,毛白杨在接种溃疡病菌后,H2O2摩尔质量浓度显著高于北京杨,在接种72 h时,H2O2摩尔质量浓度达到最大值(737.52 mol/g),此时对杨树韧皮部进行H2O2亚细胞化学定位,发现抗病毛白杨有大量H2O2-CeCl3沉淀颗粒,且增加的沉淀主要起源于细胞壁;两种杨树接种溃疡病菌后的APX和POD酶活性,毛白杨的变幅显著高于北京杨,且APX和POD的活性变化与寄主抗病性呈正相关;RT-PCR研究表明,溃疡病菌诱导了两种互作体系中杨树APX和POD基因的表达。实验结果表明不同抗性杨树接种溃疡病菌后,其体内H2O2、APX和POD表达差异与其抗性密切相关,H2O2摩尔质量浓度的积累程度对杨树抗病性具有重要的作用。
The contents of hydrogen peroxide （ H2O2 ） and the dynamic changes of POD and APX activities in resistant Populus tomentosa and susceptible P. xbeijingensis inoculated with Botryosphaeria dothidea were analyzed to study the relationship between activity differences of H2O2 and related enzymes. Results showed that the content of H2O2 in P. tomentosa was obviously higher than that in P. beifingensis at the early stage of inoculation. After 72 hours of inoculation, H2O2 content reached its maximum （757.52 mol/g）. H2O2 in poplar plant phloem was detected by cytochemical method with CeC13 stai- ning, which suggested that there were a large number of H2O2-CeC13 precipitate particles on both sides of cell wall of P. tomentosa phloem at 72 h after inoculation. After inoculation with B. dothidea, the activity amplitudes of APX and POD of P. tomentosa were significantly higher than those of P. xbeifingensis. The activities of APX and POD were positively correlated with poplar resistance. The results indicated that APX and POD were induced by B. dothidea in all interactions. The dynamic changes of H2O5 , APX and POD in different resistant poplar trees inoculated with B. dothidea were closely related to their resistances, and the accumulation level of H2O2 in the poplars had an important influence on the resistance of poplars to B. dothidea.