文献类型：期刊文献

英文题名：Microbial denitrification dominates nitrate losses from forest ecosystems

作者：Fang, Yunting[1,2] Koba, Keisuke[2] Makabe, Akiko[2] Takahashi, Chieko[2] Zhu, Weixing[3] Hayashi, Takahiro[2] Hokari, Azusa A.[2] Urakawa, Rieko[4] Bai, Edith[1] Houlton, Benjamin Z.[5] Xi, Dan[1] Zhang, Shasha[1] Matsushita, Kayo[2] Tu, Ying[1] Liu, Dongwei[1] Zhu, Feifei[1] Wang, Zhenyu[1] Zhou, Guoyi[6] Chen, Dexiang[7] Makita, Tomoko[2] Toda, Hiroto[2] Liu, Xueyan[2] Chen, Quansheng[1,8] Zhang, Deqiang[6] Li, Yide[7] Yoh, Muneoki[2]

第一作者：Fang, Yunting

通信作者：Koba, K[1]

机构：[1]Chinese Acad Sci, Inst Appl Ecol, State Key Lab Forest & Soil Ecol, Shenyang 110164, Peoples R China;[2]Tokyo Univ Agr & Technol, Inst Agr, Fuchu, Tokyo 1838509, Japan;[3]SUNY Binghamton, Dept Biol Sci, Binghamton, NY 13902 USA;[4]Univ Tokyo, Grad Sch Agr & Life Sci, Tokyo 1138657, Japan;[5]Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA;[6]Chinese Acad Sci, South China Bot Garden, Key Lab Vegetat Restorat & Management Degraded Ec, Guangzhou 510650, Guangdong, Peoples R China;[7]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[8]Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China

年份：2015

卷号：112

期号：5

起止页码：1470-1474

外文期刊名：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

收录：;Scopus(收录号：2-s2.0-84922374146);WOS:【SCI-EXPANDED(收录号:WOS:000349087700062)】；

基金：We thank X. M. Fang, M. Kuroiwa, and S. Y. Fan for field sampling or help during laboratory analysis. This work was financially supported by the Japan Society for the Promotion of Science [JSPS; Funding Program for Next Generation World-Leading Researchers (GS008) and Grants-in-Aid for Scientific Research (26252020)]; the National Natural Science Foundation of China (Grant 31370464); the State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences (Grant Y1SRC211S1); and a grant-in-aid for Scientific Research from the JSPS (Grant 21310008). B.Z.H. was supported by National Science Foundation Grant DEB-1150246. Y. F. and X. L. were also supported by the JSPS, with a Postdoctoral Fellowship for Foreign Researchers and a Grant-in-Aid for JSPS Fellows (Grants 20-08421 and 21-09318, respectively).

语种：英文

外文关键词：denitrification; nitrate isotopes; nitrogen cycling; forested watersheds

摘要：Denitrification removes fixed nitrogen (N) from the biosphere, thereby restricting the availability of this key limiting nutrient for terrestrial plant productivity. This microbially driven process has been exceedingly difficult to measure, however, given the large background of nitrogen gas (N-2) in the atmosphere and vexing scaling issues associated with heterogeneous soil systems. Here, we use natural abundance of N and oxygen isotopes in nitrate (NO3-) to examine dentrification rates across six forest sites in southern China and central Japan, which span temperate to tropical climates, as well as various stand ages and N deposition regimes. Our multiple stable isotope approach across soil to watershed scales shows that traditional techniques underestimate terrestrial denitrification fluxes by up to 98%, with annual losses of 5.6-30.1 kg of N per hectare via this gaseous pathway. These N export fluxes are up to sixfold higher than NO3- leaching, pointing to widespread dominance of denitrification in removing NO3- from forest ecosystems across a range of conditions. Further, we report that the loss of NO3- to denitrification decreased in comparison to leaching pathways in sites with the highest rates of anthropogenic N deposition.