文献类型：期刊文献

英文题名：Effects of drought and rehydration on root gene expression in seedlings of Pinus massoniana Lamb.

作者：Chen, Xinhua[1,2,3,4,5,6] Chen, Hu[4,5,6] Xu, Huilan[4,5,6] Li, Mei[1,2] Luo, Qunfeng[4,5,6] Wang, Ting[4,5,6] Yang, Zhangqi[4,5,6] Gan, Siming[1,2,4,5,6]

第一作者：Chen, Xinhua

通信作者：Gan, SM[1];Gan, SM[2];Yang, ZQ[3];Gan, SM[3];Yang, ZQ[4];Gan, SM[4];Yang, ZQ[5];Gan, SM[5]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Xiangshan Rd, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, Key Lab State Forestry Adm Trop Forestry Res, 682 Guangshan Rd 1, Guangzhou 510520, Peoples R China;[3]Nanjing Forestry Univ, Coll Forestry, 159 Longpan Rd, Nanjing 210037, Peoples R China;[4]State Forestry Adm, Engn Res Ctr Masson Pine, 23 Yongwu Rd, Nanning 530002, Peoples R China;[5]Engn Res Ctr Masson Pine Guangxi, 23 Yongwu Rd, Nanning 530002, Peoples R China;[6]Guangxi Forestry Res Inst, Guangxi Key Lab Super Timber Trees Resource Culti, 23 Yongwu Rd, Nanning 530002, Peoples R China

年份：2023

卷号：43

期号：9

起止页码：1619-1640

外文期刊名：TREE PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001125216000001)】；

基金：This work was supported by Guangxi Natural Science Foundation (2019GXNSFDA245033), Bagui Young Scholars Programme (to H.C.), Bagui Scholars Programme (2019A26, to Z.Y.), Department of Science and Technology of Guangxi Zhuang Autonomous Region, China (GuikeAD19254004) and National Natural Science Foundation of China (32060348).

语种：英文

外文关键词：abiotic stress; full-length transcriptome; Masson pine; RNA-seq; root

摘要：The mechanisms underlying plant response to drought involve the expression of numerous functional and regulatory genes. Transcriptome sequencing based on the second- and/or third-generation high-throughput sequencing platforms has proven to be powerful for investigating the transcriptional landscape under drought stress. However, the full-length transcriptomes related to drought responses in the important conifer genus Pinus L. remained to be delineated using the third-generation sequencing technology. With the objectives of identifying the candidate genes responsible for drought and/or rehydration and clarifying the expression profile of key genes involved in drought regulation, we combined the third- and second-generation sequencing techniques to perform transcriptome analysis on seedling roots under drought stress and rewatering in the drought-tolerant conifer Pinus massoniana Lamb. A sum of 294,114 unique full-length transcripts were produced with a mean length of 3217 bp and N50 estimate of 5075 bp, including 279,560 and 124,438 unique full-length transcripts being functionally annotated and Gene Ontology enriched, respectively. A total of 4076, 6295 and 18,093 differentially expressed genes (DEGs) were identified in three pair-wise comparisons of drought-treatment versus control transcriptomes, including 2703, 3576 and 8273 upregulated and 1373, 2719 and 9820 downregulated DEGs, respectively. Moreover, 157, 196 and 691 DEGs were identified as transcription factors in the three transcriptome comparisons and grouped into 26, 34 and 44 transcription factor families, respectively. Gene Ontology enrichment analysis revealed that a remarkable number of DEGs were enriched in soluble sugar-related and cell wall-related processes. A subset of 75, 68 and 97 DEGs were annotated to be associated with starch, sucrose and raffinose metabolism, respectively, while 32 and 70 DEGs were associated with suberin and lignin biosynthesis, respectively. Weighted gene co-expression network analysis revealed modules and hub genes closely related to drought and rehydration. This study provides novel insights into root transcriptomic changes in response to drought dynamics in Masson pine and serves as a fundamental work for further molecular investigation on drought tolerance in conifers.