文献类型：期刊文献

英文题名：Expression of Multiple Resistance Genes Enhances Tolerance to Environmental Stressors in Transgenic Poplar (Populus x euramericana 'Guariento')

作者：Su, Xiaohua[1] Chu, Yanguang[1] Li, Huan[1] Hou, Yingjie[1] Zhang, Bingyu[1] Huang, Qinjun[1] Hu, Zanmin[2] Huang, Rongfeng[3] Tian, Yingchuan[4]

第一作者：苏晓华

通信作者：Su, XH[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing, Peoples R China;[2]Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China;[3]Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100193, Peoples R China;[4]Chinese Acad Sci, Inst Microbiol, Beijing, Peoples R China

年份：2011

卷号：6

期号：9

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000294803100048)】；

基金：This work was financially supported by the project from the Ministry of Agriculture of China for transgenic research (2009ZX08009-098B) and the Public Benefit Research Foundation from the Ministry of Finance of China (201004004). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

摘要：Commercial and non-commercial plants face a variety of environmental stressors that often cannot be controlled. In this study, transgenic hybrid poplar (Populus x euramericana 'Guariento') harboring five effector genes (vgb, SacB, JERF36, BtCry3A and OC-I) were subjected to drought, salinity, waterlogging and insect stressors in greenhouse or laboratory conditions. Field trials were also conducted to investigate long-term effects of transgenic trees on insects and salt tolerance in the transformants. In greenhouse studies, two transgenic lines D5-20 and D5-21 showed improved growth, as evidenced by greater height and basal diameter increments and total biomass relative to the control plants after drought or salt stress treatments. The improved tolerance to drought and salt was primarily attributed to greater instantaneous water use efficiency (WUEi) in the transgenic trees. The chlorophyll concentrations tended to be higher in the transgenic lines under drought or saline conditions. Transformed trees in drought conditions accumulated more fructan and proline and had increased Fv/Fm ratios (maximum quantum yield of photosystem II) under waterlogging stress. Insect-feeding assays in the laboratory revealed a higher total mortality rate and lower exuviation index of leaf beetle [Plagiodera versicolora (Laicharting)] larvae fed with D5-21 leaves, suggesting enhanced insect resistance in the transgenic poplar. In field trials, the dominance of targeted insects on 2-year-old D5-21 transgenic trees was substantially lower than that of the controls, indicating enhanced resistance to Coleoptera. The average height and DBH (diameter at breast height) of 2.5-year-old transgenic trees growing in naturally saline soil were 3.80% and 4.12% greater than those of the control trees, but these increases were not significant. These results suggested that multiple stress-resistance properties in important crop tree species could be simultaneously improved, although additional research is needed to fully understand the relationships between the altered phenotypes and the function of each transgene in multigene transformants.