文献类型：期刊文献

中文题名：生长素种类和浓度对黑木相思组培苗生根诱导的影响

英文题名：Effects of Auxin Types and Concentrations on the Induction of Adventitious Roots in Acacia melanoxylon Tissue-cultured Seedlings

作者：张志威[1,2] 胡冰[2] 陈金辉[1] 曾炳山[2] 陆钊华[2]

第一作者：张志威

机构：[1]海南大学热带农林学院(农业农村学院、乡村振兴学院),海南海口570228;[2]中国林业科学研究院热带林业研究所/热带林业研究国家林草局重点实验室,广东广州510520

年份：2025

卷号：41

期号：5

起止页码：11-18

中文期刊名：林业与环境科学

外文期刊名：Forestry and Environmental Science

基金：国家重点研发计划子课题(2022YFD2200205-1)。

语种：中文

中文关键词：黑木相思;组织培养;生根诱导;生长素;条件优化

外文关键词：Acacia melanoxylon;tissue culture;rooting induction;auxin;condition optimization

分类号：S723.1

摘要：为探讨生长素对黑木相思Acacia melanoxylon组培苗不定根的诱导效果,提升其工厂化育苗的生产效率,以黑木相思良种SR14组培苗丛芽为材料,以1/2改良MS为基本培养基,分别添加低(0.3 mg·L^(-1))、中(0.9 mg·L^(-1))、高(1.5 mg·L^(-1))浓度的吲哚乙酸(IAA)、吲哚丁酸(IBA)、2,4-二氯苯氧乙酸(2,4-D)、α-萘乙酸(NAA)和生根粉(ABT-1),培养30 d后,统计不定根的数量、长度等生长指标,比较不同生长素或生长素类似物对SR14生根诱导的影响;并对优选出的IAA和ABT-1分别设置5个浓度梯度,进行单一使用时最佳浓度的筛选;然后,将最佳浓度的IAA和ABT-1分别与低、中、高浓度的IBA或NAA组合使用,比较其对SR14的生根诱导效果。结果得出,单独使用1.5 mg·L^(-1) IAA诱导SR14不定根时生根率最高(78.31%),单独使用2.7 mg·L^(-1) ABT-1的诱导效果次之(65.97%);组合使用两种生长素或生长素类似物诱导SR14不定根的效果优于单一种类,其中2.7 mg·L^(-1) ABT-1+1.5 mg·L^(-1) IBA诱导SR14不定根的生根率最高(82.00%),平均生根数最多(6.28条),而2.7 mg·L^(-1) ABT-1+0.9 mg·L^(-1) IBA诱导时平均根最长(12.33 cm);形态学观察表明,不定根形成于茎基部切口处褐化组织上方,逐渐发育为“奶嘴”状结构并最终伸长形成根系。研究优化了黑木相思SR14无性系不定根的诱导条件,明确了生长素种类、浓度及配比对黑木相思组培苗不定根诱导的影响。
To investigate the induction effects of auxin on adventitious roots in Acacia melanoxylon tissue-cultured seedlings and to improve the production efficiency of its industrialized seedling propagation,the cluster shoots of the superior A.melanoxylon clone SR14 tissue-cultured seedlings were used as materials.With 1/2 modified MS as the basal medium,low(0.3 mg·L^(-1)),medium(0.9 mg·L^(-1)),and high(1.5 mg·L^(-1))concentrations of indole-3-acetic acid(IAA),indole-3-butyric acid(IBA),2,4-dichlorophenoxy-acetic acid(2,4-D),α-naphthaleneacetic acid(NAA),and rooting powder(ABT-1)were added respec-tively.After 30 days of culture,growth indicators such as the number and length of adventitious roots were sta-tistically analyzed to compare the effects of different auxins or auxin analogues on the rooting induction of SR14.For the selected IAA and ABT-1,five concentration gradients were set up respectively to screen the optimal concentration for single use.Then,the optimal concentrations of IAA and ABT-1 were combined with low,medium,and high concentrations of IBA or NAA,respectively,to compare their effects on the rooting induc-tion of SR14.The results showed that when 1.5 mg·L^(-1) IAA was used alone,the rooting rate of adventitious roots in SR14 was the highest(78.31%),followed by 2.7 mg·L^(-1) ABT-1(65.97%).The effect of com-bining two auxins or auxin analogues on inducing adventitious roots in SR14 was better than that of a single kind.Among the combinations,2.7 mg·L^(-1) ABT-1+1.5 mg·L^(-1) IBA resulted in the highest rooting rate(82.00%)and the largest average number of roots(6.28),while 2.7 mg·L^(-1) ABT-1+0.9 mg·L^(-1) IBA in-duced the longest average root length(12.33 cm).Morphological observations showed that adventitious roots formed above the browned tissue at the basal cut of the stem,gradually developed into a"nipple-like"structure and eventually elongated into a root.This study optimized the induction conditions for adventitious roots in the A.melanoxylon SR14 clone,and clarified the effects of auxin types,concentrations,and combinations on the induction of adventitious roots in A.melanoxylon tissue-cultured seedlings.