文献类型：期刊文献

中文题名：Allelic variation in the coumarate 3-hydroxylase gene associated with wood properties of Catalpa fargesii Bur.

作者：Nan Lu[1] Fang Mei[1] Zhi Wang[1] Nan Wang[1] Yao Xiao[1] Lisheng Kong[2] Guanzheng Qu[3] Wenjun Ma[1] Junhui Wang[1]

第一作者：卢楠

机构：[1]Key Laboratory of Tree Genetics and Breeding,Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,People’s Republic of China;[2]Department of Biology,Centre for Forest Biology,University of Victoria,3800 Finnerty Road,Victoria,BC,Canada;[3]State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,People’s Republic of China

年份：2021

卷号：32

期号：6

起止页码：2517-2529

中文期刊名：林业研究：英文版

外文期刊名：Journal of Forestry Research

收录：CSTPCD;;Scopus;CSCD:【CSCD2021_2022】；PubMed;

基金：This work was supported by the National Key Research and Development Program of China[No.2017YFD0600201];Forestry Industry Research Special Funds for Public Welfare Projects[No.201404101].

语种：英文

中文关键词：Catalpa fargesii;Coumarate 3-hydroxylase;Haplotype-based association analyses;Linkage disequilibrium;Single-nucleotide polymorphisms;Wood properties

分类号：S781

摘要：Coumarate 3-hydroxylase(C3h)genes participate in the synthesis of lignin and may affect the properties of wood that are important for its commercial value.A better understanding of the natural variation in C3h genes and their associations to wood properties is required to effectively improve wood quality.We used a candidate gene-based association mapping approach to identify CfC3h allelic variants associated with traits that affect the wood properties of Catalpa fargesii.We first isolated the full-length CfC3h cDNA(1825 bp),which was expressed at relatively high levels in xylem according to real time-polymerase chain reaction.In totally,17 common single-nucleotide polymorphisms(minor allele frequency>5%)were identified through cloning and sequencing the CfC3h locus from a mapping population(including 88 unrelated natural C.fargesii individuals collected from main distribution area).Nucleotide diversity and linkage disequilibrium(LD)in CfC3h indicate that CfC3h has low nucleotide diversity(π_(t)=0.0031 andθ_(w)=0.0103)and relatively low LD(within 1800 bp;r^(2)≥0.1).An association analysis identified eight common single-nucleotide polymorphisms(SNPs)(false discovery rate,Q<0.10)and ten haplotypes(Q<0.10)associated with wood properties,explaining 4.92-12.09%of the phenotypic variance in an association population consisted of 125 unrelated natural individuals(The 88 individuals from the mapping population were comprised in the association population).Our study would provide new insight into C3h gene affecting wood quality,and the SNP markers identified would have potential applications in marker-assisted breeding in the future.