文献类型：期刊文献

英文题名：Patterns of molecular evolution and predicted function in thaumatin-like proteins of Populus trichocarpa

作者：Zhao, Jia Ping[1,2,3] Su, Xiao Hua[2]

第一作者：Zhao, Jia Ping

通信作者：Zhao, JP[1]

机构：[1]Agr Univ Hebei, Coll Forestry, Baoding 071001, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[3]Beijing Forestry Univ, Minist Educ, Key Lab Silviculture & Conservat, Beijing 100082, Peoples R China

年份：2010

卷号：232

期号：4

起止页码：949-962

外文期刊名：PLANTA

收录：;Scopus(收录号：2-s2.0-77955842087);WOS:【SCI-EXPANDED(收录号:WOS:000281055200014)】；

基金：This work was financially supported by National Natural Science Foundation of China (No. 30872048), and Chinese Natural Science & Technology Resources Sharing Programs (No. 2005DKA21207-14) and National Science & Technology Basic Program of China (No. 2009FY210100).

语种：英文

外文关键词：Antifungal activity; Glucanase activity; Homology modeling; Molecular evolution; Positive selection

摘要：Some pathogenesis-related proteins (PR proteins) are subject to positive selection, while others are under negative selection. Here, we report the patterns of molecular evolution in thaumatin-like protein (TLP, PR5 protein) genes of Populus trichocarpa. Signs of positive selection were found in 20 out of 55 Populus TLPs using the likelihood ratio test and ML-based Bayesian methods. Due to the connection between the acidic cleft and the antifungal activity, the secondary structure and three-dimensional structure analyses predicted antifungal activity beta-1,3-glucanase activities in these TLPs. Moreover, the coincidence with variable basic sites in the acidic cleft and positively selected sites suggested that fungal diseases may have been the main environmental stress that drove rapid adaptive evolution in Populus.