文献类型：期刊文献

中文题名：多年生黑麦草愈伤组织航天搭载后再生株系的抗旱性研究

英文题名：Drought resistance analysis of regenerated plants from calli of Lolium perenne carried by the Shenzhou No.7 spaceship

作者：彭丽梅[1,2] 曹丽[1,3] 韩蕾[1] 钱永强[1] 孙振元[1]

第一作者：彭丽梅

机构：[1]中国林业科学研究院林业研究所/国家林业局林木培育重点实验室,北京100091;[2]棕榈园林股份有限公司,上海200433;[3]延边大学农学院,吉林延吉133002

年份：2015

卷号：0

期号：1

起止页码：64-71

中文期刊名：草业学报

外文期刊名：Acta Prataculturae Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家“863”计划项目(2007AA100105,2009AA102109)资助

语种：中文

中文关键词：多年生黑麦草;空间诱变;胚性愈伤组织;抗旱性

外文关键词：Lolium perenne; space treatment; embryogenic calli; drought resistance

分类号：S688.4

摘要：利用"神舟七号"飞船搭载多年生黑麦草品种(Derby)的胚性愈伤组织经空间诱变后,从搭载再生株系(SP)群体中获得了3个抗旱变异株系。自然干旱条件下,对筛选出的3个抗旱变异株系进行了生理生化分析,结果表明,随自然干旱时间的延长,3个抗旱变异株系均表现出叶片相对含水量和叶绿素含量下降、SOD活性先升后降、丙二醛含量及游离脯氨酸含量上升等特征;不同株系间各指标的变化幅度存在差异,通过上述5项指标综合评价后初步认为,3个抗旱变异株系的抗旱性顺序为SP-64>SP-119>SP-17,与表征永久萎蔫系数反映的结果一致,表明筛选出的抗旱变异株系具有抗旱生理基础。以上结果说明,空间诱变能诱发多年生黑麦草抗旱性的变异;获得的抗旱变异株系为选育抗旱草坪草品种提供了新的种质资源。
Three mutants with high drought tolerance （SP-64, SP-119, SP-17） have been screened from plants regenerated from the embryogenic calli of the LoZium perenne variety Derby, which were carried aboard by the Shenzhou No. 7 spaceship. This paper presents the results of an evaluation of the physiological responses to drought treatment for these 3 mutants. As drought duration increased, the relative water content and chloro phyll content of the mutants decreased, whereas malondialdehyde （MDA） and soluble proline content in creased. However, the 3 mutants~ superoxide dismutase （SOD） activities at first decreased and then increased. The variation ranges of indices were different for each mutation. A comprehensive analysis of these indices sug gests that drought tolerance decreased in the order of SP-64, SP-119 and SP-17, which is consistent with the results of the apparent permanent wilting index （APWI）. These results indicate that space mutation could in duce the mutagenesis of drought resistance and so provide new germplasm resources for the breeding of turfgrass varieties.