文献类型：期刊文献

英文题名：Analysis of the leaf methylomes of parents and their hybrids provides new insight into hybrid vigor in Populus deltoides

作者：Gao, Ming[1,2] Huang, Qinjun[1,2] Chu, Yanguang[1,2] Ding, Changjun[1,2] Zhang, Bingyu[1,2] Su, Xiaohua[1,2]

第一作者：Gao, Ming

通信作者：Su, XH[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China

年份：2014

卷号：15

外文期刊名：BMC GENETICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000345651700010)】；

基金：The publication charges this article were funded by the National Key Technology Research and Development Program for the Twelfth Five-Year Plan of China (Grant No. 2012BAD01B03) and National Key Technology Research and Development Program for the Eleventh Five-Year-Plan of China (Grant No. 2006BAD01A15).

语种：英文

摘要：Background: Plants with heterosis/hybrid vigor perform better than their parents in many traits. However, the biological mechanisms underlying heterosis remain unclear. To investigate the significance of DNA methylation to heterosis, a comprehensive analysis of whole-genome DNA methylome profiles of Populus deltoides cl.'55/65' and '10/17' parental lines and their intraspecific F1 hybrids lines was performed using methylated DNA immunoprecipitation (MeDIP) and high-throughput sequencing. Results: Here, a total of 486.27 million reads were mapped to the reference genome of Populus trichocarpa, with an average unique mapping rate of 57.8%. The parents with similar genetic background had distinct DNA methylation levels. F1 hybrids with hybrid vigor possessed non-additive DNA methylation level (their levels were higher than mid-parent values). The DNA methylation levels in promoter and repetitive sequences and transposable element of better-parent F1 hybrids and parents and lower-parent F1 hybrids were different. Compared with the maternal parent, better-parent F1 hybrids had fewer hypermethylated genes and more hypomethylated ones. Compared with the paternal parent and lower-parent L1, better-parent F1 hybrids had more hypermethylated genes and fewer hypomethylated ones. The differentially methylated genes between better-parent F1 hybrids, the parents and lower-parent F1 hybrids were enriched in the categories metabolic processes, response to stress, binding, and catalytic activity, development, and involved in hormone biosynthesis, signaling pathway. Conclusions: The methylation patterns of the parents both partially and dynamically passed onto their hybrids, and F1 hybrids has a non-additive mathylation level. A multidimensional process is involved in the formation of heterosis.