文献类型：期刊文献

英文题名：F-BOX and oleosin: additional target genes for future metabolic engineering in tung trees?

作者：Chen, Yicun[1] Zhou, Guan[1] Wang, Yangdong[1] Xu, Lingna[1]

第一作者：陈益存

通信作者：Wang, YD[1]|[a0005f34f1ecf07bb8f24]汪阳东;

机构：[1]Chinese Acad Foresty, Res Inst Subtrop Foresty, Fuyang 311400, Peoples R China

年份：2010

卷号：32

期号：3

起止页码：684-686

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20104013269794);Scopus(收录号：2-s2.0-77957243154);WOS:【SCI-EXPANDED(收录号:WOS:000283962300079)】；

基金：This work was financially supported by the Zhejiang Province Natural Science Foundation of China (2009C32107) and the State Forestry Administration Fund, PR China (2009-4-23).

语种：英文

外文关键词：Tung oil; cDNA library; Expressed sequence tags; DGAT2

摘要：Tung oil, produced from seeds of the rung tree (Vernicia fordii), is a valuable industrial oil. Genetic engineering can improve the oil content, making it more suitable for use in biodiesel, and the pivotal role of DGAT2 in triacylglycerol synthesis has been extensively studied. Our data indicated, however, that DGAT2 expression decreases rapidly during the late period of oil accumulation, which raises the possibility that other key genes might play important roles in triacylglycerol synthesis. Construction of a tung kernel cDNA library and related biochemical analyses was carried out. Notably, F-BOX genes and oleosin-coding genes responsible for oil body structure were the most abundant ESTs in our cDNA library. All the results indicate that F-BOX and oleosin might be actively involved in rung lipid metabolism and serve as additional candidate genes for genetic engineering of the tung tree. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.