文献类型：期刊文献

英文题名：Identification and characterization of circular RNAs during wood formation of poplars in acclimation to low nitrogen availability

作者：Liu, Huimin[1] Yu, Wanwen[2] Wu, Jiangting[3] Li, Zhuorong[3] Li, Hui[4] Zhou, Jing[3] Hu, Jingjing[5] Lu, Yan[3]

通信作者：Lu, Y[1]|[a000526d820ed3b9b037e]逯岩;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Nontimber Forest Germplasm Enhancement &, Nontimber Forest Res & Dev Ctr, Zhengzhou 450003, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Silviculture Natl Forestry & Grassland Ad, Res Inst Forestry, Beijing 100091, Peoples R China;[4]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Trop Forestry, Guangzhou 510000, Peoples R China;[5]Inertia Shanghai Biotechnol Co Ltd, Shanghai 200335, Peoples R China

年份：2020

卷号：251

期号：2

外文期刊名：PLANTA

收录：;Scopus(收录号：2-s2.0-85077703878);WOS:【SCI-EXPANDED(收录号:WOS:000512207100001)】；

基金：This study was jointly supported by the Key Forestry Public Welfare Project (201504105), the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (No. CAFYBB2016QB005), the Jiangsu Agriculture Science and Technology Innovation Fund (JASTIF) (No. CX(16)1005), and the National Natural Science Foundation of China (No. 31500507).

语种：英文

外文关键词：ceRNA networks; Circular RNA; Low nitrogen; Populus; Secondary xylem

摘要：Main conclusion Circular RNA (circRNA) identification and expression profiles, and construction of circRNAs-miRNAs-mRNAs networks indicates that circRNAs are involved in wood formation of poplars in acclimation to low nitrogen availability. Circular RNAs (circRNAs) are covalently closed non-coding RNAs that play pivotal roles in various biological processes. However, circRNAs' roles in wood formation of poplars in acclimation to low nitrogen (N) availability are currently unknown. Here, we undertook a systematic identification and characterization of circRNAs in the wood of Populus x canescens exposed to either 50 (low N) or 500 (normal N) mu M NH4NO3 using rRNA-depleted RNA-sequencing. A total of 2,509 unique circRNAs were identified, and 163 (ca. 6.5%) circRNAs were significantly differentially expressed (DE) under low N condition. We observed a positive correlation between the expression patterns of DE circRNAs and their hosting protein-coding genes. Moreover, circRNAs-miRNAs-mRNAs' networks were identified in the wood of poplars under low N availability. For instance, upregulated several circRNAs, such as circRNA1226, circRNA 1732, and circRNA392 induced increases in nuclear factor Y, subunit A1-A (NFYA1-A), NFYA1-B, and NFYA10 transcript levels via the mediation of miR169b members, which is in line with reduced xylem width and cell layers of the xylem in the wood of low N-supplied poplars. Upregulation of circRNA1006, circRNA1344, circRNA1941, circRNA901, and circRNA146 caused increased transcript level of MYB61 via the mediation of a miR5021 member, corresponding well to the higher lignin concentration in the wood of low N-treated poplars. Overall, these results indicated that DE circRNAs play an essential role in regulating gene expression via circRNAs-miRNAs-mRNAs' networks to modulate wood anatomical and chemical properties of poplars in acclimation to low N availability.