文献类型：期刊文献

英文题名：Comprehensive genome-wide identification and functional analysis of the GT8 gene family in Eucalyptus Grandis

作者：Jie, Yufan[1] Liu, Liwan[1] Li, Linsi[1] Wu, Ai-Min[1] Fan, Chunjie[2] Liu, Siwen[1,3]

第一作者：Jie, Yufan

通信作者：Liu, SW[1];Fan, CJ[2];Liu, SW[3]

机构：[1]South China Agr Univ, Coll Forestry & Landscape Architectures, Guangdong Key Lab Innovat Dev & Utilizat Forest Pl, Guangzhou, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, State Key Lab Tree Genet & Breeding, Key Lab State Forestry & Grassland Adm Trop Forest, Guangzhou, Peoples R China;[3]Shaoguan Univ, Guangdong Prov Key Lab Utilizat & Conservat Food &, Shaoguan, Peoples R China

年份：2025

卷号：16

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-105009835348);WOS:【SCI-EXPANDED(收录号:WOS:001521780000001)】；

基金：The author(s) declare that financial support was received for the research and/or publication of this article. This work was funded by China Postdoctoral Science Foundation (Certificate Number: 2024M750964) and the Guangdong Basic and Applied Basic Research Foundation (2024A1515011209).

语种：英文

外文关键词：glycosyltransferase; GT8; gene family; Eucalyptus grandis; functional analysis

摘要：Introduction The Glycosyltransferase 8 (GT8) family is critically involved in plant cell wall synthesis, yet exhibits significant functional divergence among its members. Despite its importance, systematic characterization of GT8 genes in woody plants remains limited. This study aims to comprehensively analyze the GT8 gene family in Eucalyptus grandis to elucidate its role in cell wall biosynthesis.Methods We employed bioinformatics tools to mine the E. grandis whole-genome database. A systematic analysis was conducted, including phylogenetic classification, assessment of physicochemical properties, subcellular localization prediction, gene structure annotation, chromosome mapping, and cis-acting element identification in promoter regions.Results Fifty-two GT8 family members were identified and classified into four subfamilies: GAUT, GATL, GolS, and PGSIP. Protein molecular weights ranged from 15.75 to 185.00 kD (mean: 49.08 kD). Genes were dispersed across all chromosomes except chromosomes 3 and 7. Promoter analysis revealed ubiquitous hormone-responsive cis-elements and prevalent light-responsive elements. Phylogenetic inference suggested that EgGUX02 and EgGUX04 may mediate glucuronic acid (GlcA) incorporation into xylan side chains, while EgGAUT1 and EgGAUT12 are likely direct contributors to xylan and pectin biosynthesis.Discussion This study provides the first genome-wide functional annotation of the GT8 family in E. grandis, revealing subfamily-specific roles in cell wall polymer synthesis. The enrichment of stress- and hormone-responsive promoter elements implies regulatory complexity in cell wall remodeling. Our findings establish a foundation for targeted manipulation of xylan and pectin biosynthesis in woody plants, with potential applications in biomass engineering.