文献类型：期刊文献

英文题名：Diverse roles for a class II BPC gene in Camellia japonica through tissue-specific regulation of gene expression

作者：Yu, Yifan[1,2] Chu, Xian[1,2] Ma, Xianjin[1,2] Huang, Mingchuan[1] Hu, Zhikang[1] Li, Sijia[1,2] Yin, Hengfu[1,2]

第一作者：Yu, Yifan

机构：[1] State Key Laboratory of Tree Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang, 311400, China; [2] Zhejiang Key Laboratory of Forest Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang, 311400, China

年份：2025

卷号：311

外文期刊名：International Journal of Biological Macromolecules

收录：EI(收录号：20251918384981);Scopus(收录号：2-s2.0-105004413235)

语种：英文

外文关键词：Binding sites - Gene expression regulation - Gene therapy - Transcription factors

摘要：Basic Pentacysteine (BPC) proteins are plant-specific transcription factors that bind to GAGA dinucleotide repeats in target gene promoters. Different classes of BPC proteins are able to form complexes and recruit chromatin-modifying proteins to regulate target gene expression. However, the mechanisms by which the relationships among BPC members regulate tissue-specific gene expression remain unclear. In this study, we investigated the tissue-specific functions and mechanisms of CjBPC5, a class II BPC gene from Camellia japonica. The ectopic overexpression of CjBPC5 induced pleiotropic morphological defects in the leaves and floral tissues. Global gene expression profiling and DNA binding-site analyses showed that CjBPC5 regulated tissue-specific expression of several downstream genes by binding to the GAGA-rich binding sites in their promoters. Furthermore, we identified DNA motifs in the BPC binding sites that were critical for regulating tissue-specific gene expression and various developmental processes via transcription factor binding. Chromatin immunoprecipitation (ChIP) assay demonstrated direct binding of CjBPC5 to the promoter regions of EARLY FLOWERING 3 (ELF3), BEL1-LIKE HOMEODOMAIN 6 (BLH6) and BRASSINOSTEROID-6-OXIDASE 2 (BR6OX2). In conclusion, our study suggests that CjBPC5 plays a critical role in plant development by interacting with specific transcription factors and regulating downstream genes in a tissue-specific manner. ? 2025 Elsevier B.V.