文献类型：期刊文献

英文题名：Synthesis and modification of novel magnetic cellulose composite microspheres for laccase immobilization

作者：Yu, Fangrui[1] Li, Na[1] Chen, Lianmei[1] Zhang, Yuying[1] Sheng, Xueru[1] Wang, Bing[1] Zhang, Jian[1] Ping, Qingwei[1] Li, Hongbin[2]

第一作者：Yu, Fangrui

通信作者：Li, N[1];Li, HB[2]

机构：[1]Dalian Polytech Univ, Liaoning Prov Key Lab Pulp & Papermaking Engn, Dalian 116034, Peoples R China;[2]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Jiangsu Prov Key Lab Biomass Energy & Mat, Nanjing 210042, Peoples R China

年份：2025

卷号：306

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20250917969621);Scopus(收录号：2-s2.0-85218850684);WOS:【SCI-EXPANDED(收录号:WOS:001437489600001)】；

基金：This work was financially supported by the Scientific Research Project of Education Department of Liaoning Province, China (LJKMZ20220885) .

语种：英文

外文关键词：Laccase immobilization; Magnetic cellulose composite microspheres; Bisphenol a (BPA) degradation

摘要：In this paper, an effective strategy to improve the stability and recovery convenience of immobilized laccase by preparing magnetic particles from cheap filter paper fibers was studied, that is, MC@Fe3O4 microparticles were prepared by simple cellulose regeneration and carboxyl group functionalization, and then immobilized laccase under mild conditions. The Box-Behnken design (BBD) was used to optimize the enzyme concentration, pH value and contact time affecting the immobilization yield. The results showed that the concentration of laccase and pH value had important effects on the immobilization yield. The thermal stability, pH stability and storage stability of immobilized laccase were higher than those of free enzyme. Most importantly, the immobilized laccase had a good degradation capacity for bisphenol A (BPA), with a degradation rate of 75.65 % in the first cycle, and the BPA removal rate remained above 35 % even after 8 consecutive reuses. This study developed a new magnetic cellulose material to immobilize laccase, and the immobilized laccase had great potential in BPA wastewater treatment.