文献类型：期刊文献

中文题名：马占相思优树组培快繁技术研究

英文题名：Tissue Culture Technique of Acacia mangium Elite Trees

作者：黄烈健[1] 陈祖旭[1] 张赛群[2] 梁日高[3]

第一作者：黄烈健

机构：[1]中国林业科学研究院热带林业研究所;[2]厦门大学生命科学学院;[3]广东省江门市林业科学研究所

年份：2012

卷号：25

期号：2

起止页码：227-230

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：国家"十一五"科技支撑计划项目(2006BAD01A15-5;2006BAD32B010-3);农业科技成果转化项目(2009GB24320482)

语种：中文

中文关键词：马占相思;优树;组培

外文关键词：Acacia mangium; elite trees; tissue culture

分类号：S723.1

摘要：相思（Acacia Mill.）类树种（俗称相思树）属含羞草科（Mimosaceae）金合欢属（Acacia Mill.）,在我国广东、海南、广西、福建、云南和江西等地均有栽培。相思树植株高大挺拔,根系发达具根瘤,具有耐干旱贫瘠及抗风的特性,是水土保持及改良土壤的优良树种;同时,相思树还是优良的用材树种,
Taking Acacia mangium elite trees as explants,the tissue culture technique system for dormant bud of 3-5 year-old elite trees was established.The system includes the germination-inducing of the dormant buds,the multiplying of shoots,the rooting of adventitious shoots,and the pre-treating and transplanting of seedlings.The medium MS ＋ Sucrose 30 g·L^-1 ＋ BA 0.5 mg·L^-1 ＋ NAA 0.1 mg·L^-1 was used for inducing the dormant buds;the medium MS ＋ BA 1.0 mg·L^-1 ＋ NAA 0.05 mg·L^-1 was used for multiplying;while the medium 1/2 MS ＋ IBA 2.0 mg·L^-1 ＋ NAA 0.5 mg·L^-1 was used for rooting.The results revealed that it was viable to producing field seedlings with micropropagation.Although the branches with dormant buds harbored many kinds of microbes and the adventitious shoots were not easy to root,20% to 30% healthy germination could be yielded and the rooting rate of adventitious shoots could be higher than 85%.Pretreated with ABT powder（rooting hormone）,both the rooting rate and survival rate of adventitious shoots were nearly 100%.