文献类型：期刊文献

英文题名：Molecular breeding of water lily: engineering cold stress tolerance into tropical water lily

作者：Yu, Cuiwei[1,2,3] Qiao, Guirong[1,3] Qiu, Wenmin[1,3] Yu, Dongbei[2] Zhou, Shirong[2] Shen, Yan[2] Yu, Guanchun[2] Jiang, Jing[1,3] Han, Xiaojiao[1,3] Liu, Mingying[1,3] Zhang, Liangsheng[4] Chen, Fei[4] Chen, Yuchu[2] Zhuo, Renying[1,3]

第一作者：Yu, Cuiwei

通信作者：Zhuo, RY[1];Chen, YC[2];Zhuo, RY[3];Chen, F[4]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Zhejiang Humanities Landscape Co Ltd, Hangzhou Tianjing Aquat Bot Garden, Hangzhou 310000, Zhejiang, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Zhejiang, Peoples R China;[4]Fujian Agr & Forestry Univ, Fujian Prov Key Lab Haixia Appl Plant Syst Biol, Minist Educ Genet Breeding & Multiple Utilizat Cr, Key Lab,State Key Lab Ecol Pest Control Fujian &, Fuzhou 350002, Fujian, Peoples R China

年份：2018

卷号：5

期号：1

外文期刊名：HORTICULTURE RESEARCH

收录：;Scopus(收录号：2-s2.0-85057610089);WOS:【SCI-EXPANDED(收录号:WOS:000452314900001)】；

基金：We thank Prof. Qiang Zhu from Fujian Agriculture and Forestry University for his careful reading of this manuscript and helpful suggestions. F.C. is supported by a grant from National Science Foundation, China (31801898), a grant from State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops (SKB2017004), and a grant from Natural Science Foundation of Fujian Province (2018J01603).

语种：英文

摘要：Water lilies (order Nymphaeales) are rich in both economic and cultural values. They grow into aquatic herbs, and are divided into two ecological types: tropical and hardy. Although tropical water lilies have more ornamental and medicinal values compared to the hardy water lily, the study and utilization of tropical water lilies in both landscaping and pharmaceutical use is greatly hindered due to their limited planting area. Tropical water lilies cannot survive the winter in areas beyond 24.3 degrees N latitude. Here, the transgenic pipeline through the pollen-tube pathway was generated for water lily for the first time. To improve cold stress tolerance of tropical water lilies, a gene encoding choline oxidase (CodA) driven by a cold stress-inducible promoter was transformed into a tropical water lily through the pollen-tube transformation. Six independent transgenic lines were tested for survival rate during two winter seasons from 2015 to 2017 in Hangzhou (30.3 degrees N latitude). PCR and southern blot detection revealed that the CodA gene had been integrated into the genome. Reverse transcription PCR showed that CodA gene was induced after cold stress treatment, and further quantitative real-time PCR revealed different expressions among six 4 lines and line 3 had the highest expression. Multiple physiological experiments showed that after cold stress treatment, both the conductivity and malondialdehyde (MDA) levels from transgenic plants were significantly lower than those of non-transgenic plants, whereas the content of betaine and the activity of superoxide dismutase, catalase, and peroxidase were higher than those from non-transgenic plants. These results suggest that expression of exogenous CodA gene significantly improved the cold stress tolerance of tropical water lilies through a wide range of physiological alterations. Our results currently expanded a six-latitude cultivating area of the tropical water lilies. These results not only illuminate the bright future for water lily breeding but will also facilitate the functional genomic studies.