文献类型：期刊文献

英文题名：Contribution of macrolactin in Bacillus velezensis CLA178 to the antagonistic activities against Agrobacterium tumefaciens C58

作者：Chen, Lin[1,3] Wang, Xinghong[1,3] Liu, Yunpeng[2]

第一作者：陈淋;Chen, Lin

通信作者：Liu, YP[1]

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China;[2]Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Key Lab Agr Microbial Resources Collect & Preserv, Minist Agr & Rural Affairs, Beijing 100081, Peoples R China;[3]Natl Permanent Sci Res Base Warm Temperate Zone F, Beijing 102300, Peoples R China

年份：2021

卷号：203

期号：4

起止页码：1743-1752

外文期刊名：ARCHIVES OF MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85099570727);WOS:【SCI-EXPANDED(收录号:WOS:000609085800003)】；

基金：This work was supported by National Natural Science Foundation of China (31700548), the Fundamental Research Funds for the Central Non-profit Research Institution of the Chinese Academy of Forestry (CAFYBB2017MA020) and National industry innovation alliance of Rosa.

语种：英文

外文关键词：Biocontrol; Bacillus velezensis; Macrolactin; Agrobacterium tumefaciens; Antagonistic

摘要：Beneficial rhizobacteria can inhibit soilborne pathogens by secreting an array of polyketides, lipopeptides and dipeptides, but the effect of polyketides on crown gall disease caused by Agrobacterium tumefaciens C58 is unclear. In this study, the antagonistic compounds of the plant growth-promoting rhizobacterium Bacillus velezensis CLA178 was sorted with different organic phases, purified by high-pressure liquid chromatography, and detected by a liquid chromatography ionization-mass spectrometry system. Macrolactins were found to be the compounds with antagonistic activity against A. tumefaciens C58. When the macrolactin synthesis pathway was disrupted, the mutant omlnA only showed slight antagonistic activity against A. tumefaciens C58. Transmission electron microscopy showed that the inhibition of C58 cell division by cell-free culture from the mutant omlnA was weaker than that by cell-free culture from CLA178. The mutant deficient in production of macrolactin showed a weaker transcription of genes involved in attachment of C58 to plant and lower biocontrol of crown gall disease in rose than the wild-type strain CLA178. The effect of macrolactins on pathogen C58 has been also confirmed by the purified macrolactins. These results reveal that macrolactins contribute to the biocontrol activity of C58 by inhibiting cell division and downregulating the transcription of chvB and chvE.