文献类型：期刊文献

英文题名：Microbial C:N:P stoichiometry and turnover depend on nutrients availability in soil: A 14C, 15N and 33P triple labelling study

作者：Chen, Jie[1,2] Seven, Jasmin[3] Zilla, Thomas[3] Dippold, Michaela A.[2] Blagodatskaya, Evgenia[3,4] Kuzyakov, Yakov[3,4,5]

第一作者：陈洁;Chen, Jie

通信作者：Chen, Jie

机构：[1] Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, 510520, China; [2] Biogeochemistry of Agroecosystems, Department of Crop Sciences, University of G?ttingen, Büsgenweg 2, G?ttingen, 37077, Germany; [3] Dept. of Soil Science of Temperate Ecosystems, Dept. of Agricultural Soil Science, University of G?ttingen, Büsgenweg 2, G?ttingen, 37077, Germany; [4] Agricultural Soil Science, Department of Crop Sciences, University of Goettingen, Büsgenweg 2, G?ttingen, 37077, Germany; [5] Agro-Technology Institute, RUDN University, Moscow, Russia

年份：2019

卷号：131

起止页码：206-216

外文期刊名：Soil Biology and Biochemistry

收录：EI(收录号：20190506439863);Scopus(收录号：2-s2.0-85060544251)

基金：This work was done in the framework of the DFG special priority program 1685 “Ecosystem nutrition: Forest strategies for limited phosphorus resources” within the project DFG KU 1184/36-1 . We greatly acknowledge funding by the German Research Foundation . We sincerely thank the China Scholarship Council for funding Jie Chen, the Center for Stable Isotopes (KOSI) in G?ttingen for fast and reliable measurement of stable isotope samples, and the Radioisotope Laboratory (LARI) for their support in handling the radioisotope tracers. We thank Huangdong Zang, Kyle Mason-Jones, Callum Banfield and Deejay Maranguit for help and support during the incubation experiment, and Karin Schmidt for measuring the solid and liquid samples for C and N. We thank the four anonymous referees' constructive comments for the improvement of the paper. We thank Soil Science Consulting ( https://soilscicon.wordpress.com ) for help in the paper preparation.

语种：英文

外文关键词：Availability - Biomass - Carbon dioxide - DNA - Ecology - Forestry - Maintenance - Metabolism - Microorganisms - Phosphorus - Soils - Stoichiometry

摘要：Microbial biomass turnover and the associated recycling of carbon (Cmic), nitrogen (Nmic) and phosphorus (Pmic) depend on their stoichiometric relationships and plays a pivotal role for soil fertility. This study examines the responses of Cmic, Nmic, Pmic, the microbial respiration rate (CO2 efflux), and the total DNA content to C and nutrient addition in forest soils with very low (Low-P) and high P (High-P) contents. Both the Low-P and High-P soils were treated with a low and high level of C, N and P (5% and 200% of Cmic, Nmic and Pmic). Phosphorus (33P) was added before the addition of C (14C) and N (15N) to investigate the potential P limitation. We hypothesized two modes of microbial biomass C and nutrient turnover: 1) maintenance through intracellular metabolisms and/or 2) microbial growth and death through necromass reutilization. In Low-P soil, the 2-day-sooner increase of Cmic and Pmic compared to the increase of CO2 efflux and DNA content after high CN input showed the rapid initial uptake of C and limiting nutrients into microbial cells. It also demonstrated a lag period before microbial growth commenced. In High-P soil, however, the CO2 efflux and DNA content increased simultaneously with increases in microbial biomass, reflecting the microbial capacity for immediate growth. Afterwards, CO2 efflux and DNA content dropped to the level before CNP addition, with a decline of Cmic and Pmic in Low-P soil and a decline of Nmic in High-P soil, suggesting a C and P limitation in Low-P soil and N limitation in High-P soil. Under low CNP addition, the microorganisms in High-P soil are ready to grow, while those in Low-P soil are mainly in maintenance mode. The microorganisms under maintenance in low-P soil can switch to growth/death mode after removing the nutrient limitation. High CNP input caused a non-homeostatic response of Cmic: Nmic: Pmic stoichiometry from 691:105:1 to 33:1:1 in Low-P soil, mainly resulting from a higher storage of the limiting elements (C and P) in microbial biomass. The ratio remained stable under low CNP addition due to the endogenous metabolism of C and nutrient at maintenance. The C and nutrient were turnovered much faster by microorganisms in the growth/death mode, confirming a key principle of ecology: the stronger the limitation by an element, the more efficiently that element is retained within an organism, and the more intensively it is reused. The triple labeling approach linked with Cmic: Nmic: Pmic stoichiometry helped to identify the dominant maintenance and growth/death modes of microbial biomass CNP turnover in nutrient-limited and -unlimited soil. ? 2019 Elsevier Ltd